CAJ | 학술논문

目的探讨兔创伤性脑损伤(TBI)后血清抗脑抗体(AB-Ab)水平的变化及其对血脑屏障(BBB)和脑水肿的影响. 方法将45只新西兰兔按随机数字表法分为对照组(5只)、假手术组(20只)和TBI组(20只),TBI组又分为1,3,7,14 d亚组.假手术组只开骨窗不打击;TBI组建立脑单侧重型液压冲击TBI模型.分别于术后1,3,7,14d检测各组血清AB-Ab浓度、BBB通透性和脑含水量. 结果假手术组、TBI组血清AB-Ab含量、BBB通透性和脑含水量在各时相点均高于对照组(P＜0.05),TBI组血清AB-Ab含量、BBB通透性和脑含水量在3,7,14 d均高于假手术组(P＜0.05). 结论 TBI后血清中AB-Ab与BBB通透性升高和脑水肿程度呈同步性改变,提示AB-Ab可能是BBB通透性升高和脑水肿的原因之一.
목적 탐토토창상성뇌손상(TBI)후혈청항뇌항체(AB-Ab)수평적변화급기대혈뇌병장(BBB)화뇌수종적영향. 방법 장45지신서란토안수궤수자표법분위대조조(5지)、가수술조(20지)화TBI조(20지),TBI조우분위1,3,7,14 d아조.가수술조지개골창불타격;TBI조건립뇌단측중형액압충격TBI모형.분별우술후1,3,7,14d검측각조혈청AB-Ab농도、BBB통투성화뇌함수량. 결과 가수술조、TBI조혈청AB-Ab함량、BBB통투성화뇌함수량재각시상점균고우대조조(P＜0.05),TBI조혈청AB-Ab함량、BBB통투성화뇌함수량재3,7,14 d균고우가수술조(P＜0.05). 결론 TBI후혈청중AB-Ab여BBB통투성승고화뇌수종정도정동보성개변,제시AB-Ab가능시BBB통투성승고화뇌수종적원인지일.
Objective To investigate the changes of antibrain-antibody (AB-Ab) in blood serum and the effect of AB-Ab on blood-brain barrier (BBB) and cerebral edema after the traumatic brain injury (TBI) in rabbits.Methods Forty-five New Zealand rabbits were randomly divided into control group (n =5),sham operation group (fenestration only without injury,n =15) and TBI group (severe lateral fluid percussion brain injury,n =15) according to the random number table.Sham and TBI animals were subdivided at postoperative 1,3,7 and 14 days.Level of AB-Ab,BBB permeability and brain water content were determined after operation.Results At each time point,serum AB-Ab level,BBB permeability and brain water content were higher in sham operation group and TBI group than in control group (P ＜0.05).At postoperative 3,7 and 14 days,serum AB-Ab level,BBB permeability and brain water content were higher in TBI group than in sham operation group (P ＜ 0.05).Conclusion Serum level of AB-Ab is significantly increased after TBI along with synchronous changes in BBB permeability and brain water content,indicating that AB-Ab may be associated with the increase of BBB permeability and brain edema.