CAJ | 학술논문

目的：检测苏拉明对肺癌Calu-1细胞上清液诱发的淋巴管内皮细胞管腔形成能力的影响。方法应用免疫荧光染色对淋巴管内皮细胞进行鉴定；应用Matrigel立体培养法检测淋巴管内皮细胞管腔形成能力。结果苏拉明对淋巴管内皮细胞的管腔形成能力无显著影响；肿瘤上清液能够增强淋巴管内皮细胞的管腔形成能力，呈剂量依赖性；而苏拉明能够抑制肿瘤上清液诱发的淋巴管内皮细胞的管腔形成，并呈剂量依赖性。结论肿瘤上清液能够促进淋巴管内皮细胞的管腔形成，而苏拉明能够抑制这种促进作用。
목적：검측소랍명대폐암Calu-1세포상청액유발적림파관내피세포관강형성능력적영향。방법응용면역형광염색대림파관내피세포진행감정；응용Matrigel입체배양법검측림파관내피세포관강형성능력。결과소랍명대림파관내피세포적관강형성능력무현저영향；종류상청액능구증강림파관내피세포적관강형성능력，정제량의뢰성；이소랍명능구억제종류상청액유발적림파관내피세포적관강형성，병정제량의뢰성。결론종류상청액능구촉진림파관내피세포적관강형성，이소랍명능구억제저충촉진작용。
Objective To study the impact of the suramin on the tube formation induced by lung cancer Calu-1 cell supernatant. Methods Cell immunofluorescence was applied to test the lymphatic endothelial cells.Matrigel was used to test the ability of lymphatic en-dothelial cells'tube formation.Results Suramin had little effect on lymphatic endothelial cells'proliferation and the ability of tube forma-tion.Tumor supernatant fluid enhanced the ability of tube formation in a dose-dependent, while suramin inhabited this change caused by tumor supernatant fluid in a dose-dependent.Conclusions Tumor supernatant fluid could promote the tube formation of lymphatic endotheli-al cells, while suramin could inhabit the tube formation of the lymphatic endothelial cells caused by tumor supernatant fluid.