CAJ | 학술논문

目的：探讨盐霉素对低分化鼻咽癌干细胞放疗敏感性的影响。方法将鼻咽癌干细胞悬液放入标有1～24的孔板中培养，其中1～12为对照组，13～24为实验组，其中向对照组加入200μl的PBS缓冲液，向实验组加入200μl终浓度为2μmol／L的盐霉素，适宜环境中培养12 h后，两组细胞均给予6 Gy单次照射，放疗结束后，接种至培养皿中常规培养2 w，GIEMSA染色，计算细胞存活分数（SF），采用 RT-PCR及 Western 印迹法检测选取培养24 h后人鼻咽癌干细胞中Rad51、Ku70、Ku80基因及蛋白表达水平。结果实验组培养皿中细胞SF明显低于对照组（ P＜0.05）。实验组培养皿鼻咽癌干细胞Rad51、Ku70和Ku80 mRNA表达水平低于对照组（P＜0.05）。实验组培养皿鼻咽癌干细胞 Rad51、Ku70和Ku80蛋白表达水平明显低于对照组（P＜0.05）。结论盐霉素能够明显抑制人低分化鼻咽癌干细胞中 Rad51、Ku70和 Ku80表达，降低细胞存活分数，提高放疗敏感性和临床疗效。
목적：탐토염매소대저분화비인암간세포방료민감성적영향。방법장비인암간세포현액방입표유1～24적공판중배양，기중1～12위대조조，13～24위실험조，기중향대조조가입200μl적PBS완충액，향실험조가입200μl종농도위2μmol／L적염매소，괄의배경중배양12 h후，량조세포균급여6 Gy단차조사，방료결속후，접충지배양명중상규배양2 w，GIEMSA염색，계산세포존활분수（SF），채용 RT-PCR급 Western 인적법검측선취배양24 h후인비인암간세포중Rad51、Ku70、Ku80기인급단백표체수평。결과실험조배양명중세포SF명현저우대조조（ P＜0.05）。실험조배양명비인암간세포Rad51、Ku70화Ku80 mRNA표체수평저우대조조（P＜0.05）。실험조배양명비인암간세포 Rad51、Ku70화Ku80단백표체수평명현저우대조조（P＜0.05）。결론염매소능구명현억제인저분화비인암간세포중 Rad51、Ku70화 Ku80표체，강저세포존활분수，제고방료민감성화림상료효。
Objective To investigate the effects of salinomycin on radiation sensitivity of low differentiation nasopharyngeal carcino -ma stem cells.Methods Nasopharyngeal carcinoma stem cell suspension were put into orifice plate marked with 1~24, plated with1~12 were selected as the control group, with 13~24 were selected as the experimental group.200μl PBS were added into control group, 200μl salinomycin ( concentration was 2 μmol/L) were added into experimental group.Cells were cultured into suitable environment for 12 h, and were given 6 Gy single radiation therapy.After radiation therapy, cells were cultured into petri dish for 2 weeks.Cell survival fraction ( SF) was calculated by GIEMSA staining, Rad51, Ku70, Ku80 gene and protein expression were detected by RT-PCR and Western blot after cul-tured 24 h.Results SF in experimental group was lower than that of control group (P<0.05).Expression of Rad51, Ku70, Ku80 mRNA in experimental group were lower than those of control group (P<0.05).Expression of Rad51, Ku70, Ku80 protein in experimental group were lower than those of control group (P<0.05).Conclu sions Salinomycin could significantly inhibit the expressions of Rad51, Ku70 and Ku80 in low differentiation nasopharyngeal carcinoma cells, reduce the SF, improve radiation sensitivity and clinical curative effect.