CAJ | 학술논문

目的：建立一种同时测定蜜饯中7种常见合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、诱惑红、赤藓红)的固相萃取-高效液相色谱(solid-phase extraction-high performance liquid chromatography, SPE-HPLC)分析方法。方法样品经水提取后,采用 Anpelclean PA 聚酰胺 SPE 小柱净化,氮吹后定容,以甲醇和20 mmol/L乙酸铵为流动相,通过Waters 公司XBridge C18柱梯度洗脱分离,在特异性波长下进行测定。结果7种常见合成色素在0.50~10.0μg/mL范围内峰面积与其实际浓度线性相关性良好(r2≥0.9987),在0.5、2.5、5.0 mg/kg三个加标水平上回收率在74.3%~97.5%之间, RSD在0.47%~4.80%之间。检出限在0.02~0.10 mg/kg之间,定量限在0.06~0.30 mg/kg之间。结论本方法灵敏度高,分离效果好,精密度好,准确度高,适用于蜜饯中7种常见合成色素的测定。
목적：건립일충동시측정밀전중7충상견합성색소(저몽황、현채홍、연지홍、일락황、량람、유혹홍、적선홍)적고상췌취-고효액상색보(solid-phase extraction-high performance liquid chromatography, SPE-HPLC)분석방법。방법양품경수제취후,채용 Anpelclean PA 취선알 SPE 소주정화,담취후정용,이갑순화20 mmol/L을산안위류동상,통과Waters 공사XBridge C18주제도세탈분리,재특이성파장하진행측정。결과7충상견합성색소재0.50~10.0μg/mL범위내봉면적여기실제농도선성상관성량호(r2≥0.9987),재0.5、2.5、5.0 mg/kg삼개가표수평상회수솔재74.3%~97.5%지간, RSD재0.47%~4.80%지간。검출한재0.02~0.10 mg/kg지간,정량한재0.06~0.30 mg/kg지간。결론본방법령민도고,분리효과호,정밀도호,준학도고,괄용우밀전중7충상견합성색소적측정。
Objective To establish a method for simultaneous determination of 7 kinds of synthetic pigments (lemon yellow, amaranth, ponceau 4r, sunset yellow, coomassie brilliant blue, allura red and erythrosine) in preserved fruits with solid-phase extraction-high performance liquid chromatography (SPE-HPLC). Methods The homogenized samples were extracted by water, purified by Anpelclean PA- SPE column, and adjusted to constant volume after nitrogen blowing. HPLC analysis was conducted using methanol and 20 mmol/L ammonium acetate as mobile phase with a xbridge-C18 column from Waters company for gradient elution and separation. Specific wavelengths were applied for quantitative detection of the synthetic pigments in the samples. Results There was a good linearity between peak areas and concentrations of 7 kinds of synthetic pigments in the concentration range of 0.50~10.0 μg/mL (r2≥0.9987). The recovery rates fell in the scope of 74.3%~97.5% and RSDs ranged from 0.47% to 4.80% under the standard addition levels of 0.5, 2.5 and 5.0 mg/kg. The detection limits were between 0.02 and 0.10 mg/kg and the quantification limits between 0.06 and 0. 30 mg/kg. Conclusion The method has high sensitivity, good separation effect, good precision and high accuracy, thus, it’s suitable for the simultaneous determination of 7 kinds of common fruit pigments in preserved fruits.