CAJ | 학술논문

目的：建立转基因苜蓿草J101品系特异性定性PCR检测方法。方法根据转基因苜蓿草品系J1015’端外源插入片段与苜蓿草基因组 DNA 之间的邻接区序列设计引物,建立了转基因苜蓿草 J101品系特异性定性PCR检测方法，并对本方法的特异性、灵敏度进行了测定。结果建立的检测方法特异于转基因苜蓿草J101检测,检测最低DNA 浓度为(1imit of det ection,LOD)为80 pg,相当于50拷贝转基因苜蓿草J101基因组DNA。结论本研究建立的转基因苜蓿草J101品系特异性定性PCR 检测方法特异性好,灵敏度高,能够快速、准确地对转基因苜蓿草J101进行检测分析。
목적：건립전기인목숙초J101품계특이성정성PCR검측방법。방법근거전기인목숙초품계J1015’단외원삽입편단여목숙초기인조 DNA 지간적린접구서렬설계인물,건립료전기인목숙초 J101품계특이성정성PCR검측방법，병대본방법적특이성、령민도진행료측정。결과건립적검측방법특이우전기인목숙초J101검측,검측최저DNA 농도위(1imit of det ection,LOD)위80 pg,상당우50고패전기인목숙초J101기인조DNA。결론본연구건립적전기인목숙초J101품계특이성정성PCR 검측방법특이성호,령민도고,능구쾌속、준학지대전기인목숙초J101진행검측분석。
Objective To establish a event-specific qualitative PCR detection method for genetically modified (GM) alfalfa events J101. Methods The specific primer pairs based on the 5’junction sequence spanning the alfalfa DNA and inserted fragment of J101 were designed and then the PCR detection system was established. The specificity and sensitivity were analyzed. Results The qualitative PCR method was specific for GM alfalfa J101 detection, the limit of detection (LOD) were 80 pg J101 genomic DNA or 50 copies of alfalfa J101 genomic DNA. Conclusion The established event-specific PCR method for GM alfalfa J101 detection has a high specificity and good sensitivity, and is suitable for detection of J101 samples quickly and accurately.