CAJ | 학술논문

目的：为了提升实验室的食品中沙门氏菌检测能力，增强实验室竞争能力，本实验室参加了中国食品药品检定研究院组织的NIFDC-PT-010食品中沙门氏菌检出能力验证。方法利用全自动免疫检测系统(VIDAS)对能力验证中的5个样品进行快速筛查，依据GB4789.4-2010沙门氏菌检验进行血清学试验，采用16S rDNA全序列分析、全自动微生物生化鉴定系统(VITEK2)和全自动微生物基因指纹鉴定系统(RiboPrinter)对分离出的疑似菌进行鉴定。结果编号为CODE 1的样品检出阿贡纳沙门氏菌和婴儿沙门氏菌， CODE 3检出蒙得维的亚沙门氏菌， CODE 5检出鼠伤寒沙门氏菌， CODE 2和CODE 4未检出。结论5个样品测试均取得优秀的结果。
목적：위료제승실험실적식품중사문씨균검측능력，증강실험실경쟁능력，본실험실삼가료중국식품약품검정연구원조직적NIFDC-PT-010식품중사문씨균검출능력험증。방법이용전자동면역검측계통(VIDAS)대능력험증중적5개양품진행쾌속사사，의거GB4789.4-2010사문씨균검험진행혈청학시험，채용16S rDNA전서렬분석、전자동미생물생화감정계통(VITEK2)화전자동미생물기인지문감정계통(RiboPrinter)대분리출적의사균진행감정。결과편호위CODE 1적양품검출아공납사문씨균화영인사문씨균， CODE 3검출몽득유적아사문씨균， CODE 5검출서상한사문씨균， CODE 2화CODE 4미검출。결론5개양품측시균취득우수적결과。
Objective To strengthen the detection capability of Salmonella in food and improve the competition ability of laboratory, and validate the capability of microorganism detection by ability test. The laboratory participated in proficiency testing on Salmonella detection in foods of NIFDC-PT-010 organized by National Institutes for Food and Drug Control. Methods Full automatic immunoassay system (VIDAS) was used for 5 samples rapid screening, Salmonella serology test was carried out according to GB4789.4-2010. The isolated bacteria of 5 samples were identified by 16S rDNA sequence analysis, automatic microbial, biochemical identification system (VITEK2) and automatic microbial genetic fingerprint identification system (RiboPrinter). ResultsSalmonella agone and Salmonella infants were detected in CODE 1, Salmonella Montevideo was detected in CODE 3, Salmonella typhimuriumwas detected in CODE 5, and Salmonella spp. was not detected in CODE 2 and CODE 4. Conclusion The test results of 5 samples were outstanding.