化学传感器
化學傳感器
화학전감기
CHEMICAL SENSORS
2015年
1期
47-51
,共5页
免疫传感器%金-石墨烯%铁氰化镍%甲胎蛋白
免疫傳感器%金-石墨烯%鐵氰化鎳%甲胎蛋白
면역전감기%금-석묵희%철청화얼%갑태단백
immunosensor%gold-grapheme nanocomposite%nickel hexacyanoferrates nanoparticles%α-1-fetoprotein
以具有大比表面积和良好生物相容性的纳米金颗粒(AuNPs)和金-石墨烯复合物(Au-Gra)为基底,成功制备了高灵敏甲胎蛋白免疫传感器。首先在金电极表面电沉积一层纳米金，接着滴涂一层具有独特电化学活性的铁氰化镍纳米粒子(NiNPs)电子媒介体，然后滴加一层Au-Gra复合膜，最后利用Au-Gra结合甲胎蛋白抗体(anti-AFP)，从而制得甲胎蛋白免疫传感器。采用循环伏安法对电极性能进行了验证，结果表明该传感器有良好的电流响应性能，检测线性范围为0.1~100 ng/mL，检出限为0.03 ng/mL (S/N=3)。此传感器简单、快速、灵敏，可望用于临床诊断。
以具有大比錶麵積和良好生物相容性的納米金顆粒(AuNPs)和金-石墨烯複閤物(Au-Gra)為基底,成功製備瞭高靈敏甲胎蛋白免疫傳感器。首先在金電極錶麵電沉積一層納米金，接著滴塗一層具有獨特電化學活性的鐵氰化鎳納米粒子(NiNPs)電子媒介體，然後滴加一層Au-Gra複閤膜，最後利用Au-Gra結閤甲胎蛋白抗體(anti-AFP)，從而製得甲胎蛋白免疫傳感器。採用循環伏安法對電極性能進行瞭驗證，結果錶明該傳感器有良好的電流響應性能，檢測線性範圍為0.1~100 ng/mL，檢齣限為0.03 ng/mL (S/N=3)。此傳感器簡單、快速、靈敏，可望用于臨床診斷。
이구유대비표면적화량호생물상용성적납미금과립(AuNPs)화금-석묵희복합물(Au-Gra)위기저,성공제비료고령민갑태단백면역전감기。수선재금전겁표면전침적일층납미금，접착적도일층구유독특전화학활성적철청화얼납미입자(NiNPs)전자매개체，연후적가일층Au-Gra복합막，최후이용Au-Gra결합갑태단백항체(anti-AFP)，종이제득갑태단백면역전감기。채용순배복안법대전겁성능진행료험증，결과표명해전감기유량호적전류향응성능，검측선성범위위0.1~100 ng/mL，검출한위0.03 ng/mL (S/N=3)。차전감기간단、쾌속、령민，가망용우림상진단。
Based on gold nanparticles (AuNPs) and gold-grephene (Au-Gra) nanomaterials, an highly sensitive am-prometric immunosensor forα-1-fetoprotein was successfully prepared. Firstly, AuNPs were electrodeposited on the surface of bare gold electrode. Then NiNPs were dropped on the electrode and served as electrochemical redox probe, next Au-Gra was fabricated to combine anti-AFP. The electrochemical characteristics of the immunosensor were demonstrated by cyclic voltammetry (CV). The immunosensor exhibited a good linear range from 0.1 to 100 ng/mL with a detection limit of 0.03 ng/mL (S/N=3). The proposed immunosensor was simple, rapid and sensitive, and may provide a promise method for clinical detection. of AFP.
