食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
5期
1919-1923
,共5页
高效液相色谱法%苏丹红%食品?
高效液相色譜法%囌丹紅%食品?
고효액상색보법%소단홍%식품?
high?performance?liquid?chromatography%Sudan?dyes%food??
目的：建立准确可靠的高效液相色谱法测定食品中苏丹红I~IV的方法。方法用乙腈提取样品中的苏丹红,经漩涡超声离心后,将乙腈层倾倒出,用乙腈重复提取,通过凝胶色谱的净化,用液相色谱进行检测。其中凝胶色谱柱为Bio-BeadsTM S-X3 Beads,液相色谱柱为Waters C18(4.6 mm×150 mm,5μm),液相色谱流动相为乙腈-水(V：V,90：10)。结果4种苏丹红染料的检出限均能达到10μg/kg,满足国标要求。标准加入量为0.16μg/mL 浓度时,辣椒粉的回收率为92.5%~97.5%、辣椒酱的回收率为83.8%~87.5%、香肠的回收率为82.5%~98.8%,相关系数0.9995以上,且重复性良好。结论高效液相色谱法可以测定食品中苏丹红I~IV,适用于批量样品的检测。
目的：建立準確可靠的高效液相色譜法測定食品中囌丹紅I~IV的方法。方法用乙腈提取樣品中的囌丹紅,經漩渦超聲離心後,將乙腈層傾倒齣,用乙腈重複提取,通過凝膠色譜的淨化,用液相色譜進行檢測。其中凝膠色譜柱為Bio-BeadsTM S-X3 Beads,液相色譜柱為Waters C18(4.6 mm×150 mm,5μm),液相色譜流動相為乙腈-水(V：V,90：10)。結果4種囌丹紅染料的檢齣限均能達到10μg/kg,滿足國標要求。標準加入量為0.16μg/mL 濃度時,辣椒粉的迴收率為92.5%~97.5%、辣椒醬的迴收率為83.8%~87.5%、香腸的迴收率為82.5%~98.8%,相關繫數0.9995以上,且重複性良好。結論高效液相色譜法可以測定食品中囌丹紅I~IV,適用于批量樣品的檢測。
목적：건립준학가고적고효액상색보법측정식품중소단홍I~IV적방법。방법용을정제취양품중적소단홍,경선와초성리심후,장을정층경도출,용을정중복제취,통과응효색보적정화,용액상색보진행검측。기중응효색보주위Bio-BeadsTM S-X3 Beads,액상색보주위Waters C18(4.6 mm×150 mm,5μm),액상색보류동상위을정-수(V：V,90：10)。결과4충소단홍염료적검출한균능체도10μg/kg,만족국표요구。표준가입량위0.16μg/mL 농도시,랄초분적회수솔위92.5%~97.5%、랄초장적회수솔위83.8%~87.5%、향장적회수솔위82.5%~98.8%,상관계수0.9995이상,차중복성량호。결론고효액상색보법가이측정식품중소단홍I~IV,괄용우비량양품적검측。
Objective To establish an accurate and reliable determination method of Sudan I, II, III, and IV in food by HPLC. Methods The Sudan in samples were extracted with acetonitrile, after through ultrasonic, vortex and centrifugation, and the layer was poured and repeated processing with acetonitrile, and purified by GPC. The Sudan were detected by liquid chromatography. In this study, GPC column was Bio-BeadsTM S-X3 Beads, and liquid chromatographic column was Waters C18 (4.6 mm×150 mm, 5 μm), acetonitrile-water as the mobile phase was used in isocratic conditions (V:V, 90:10). Results The limit of detection (LOD) of method was 10 μg/kg, which met the requirements of the national standard. Recoveries of Sudan dyes in chilli powder ranged from 92.5% to 97.5%, chili sauce ranged from 83.8% to 87.5% and sausage ranged from 82.5% to 98.8% with a good reproducibility at a spiking level of 0.16 μg/mL. Conclusion This method was applicable for batch sample to determine Sudan I~IV.
