CAJ | 학술논문

目的：建立富含维生素 C 食品中总黄酮的定量分析方法,解决维生素 C 干扰络合法测定总黄酮的问题。方法使用Waters Sep-Pak C18固相萃取小柱对提取物进行脱脂和去除色素, D101大孔固相萃取(SPE)小柱去除维生素C,得到总黄酮洗脱液,用硝酸铝比色法进行测定,检测波长510 nm。结果总黄酮在0~48μg/mL具有良好的线性关系,相关系数为0.9998,相对标准偏差(RSD)小于5%,三个水平的添加回收率为95.31%~98.38%。考察三种不同总黄酮和维生素C比例含量的食品(Vc：总黄酮=1：1,2：1,10：1),发现随着维生素C含量增加,对总黄酮测定结果的影响越大。因此,对样品进行前处理,可以测得较为准确的总黄酮含量。结论本方法样品前处理简单、环保、快速、准确,适用于富含维生素C食品中总黄酮的含量测定。
목적：건립부함유생소 C 식품중총황동적정량분석방법,해결유생소 C 간우락합법측정총황동적문제。방법사용Waters Sep-Pak C18고상췌취소주대제취물진행탈지화거제색소, D101대공고상췌취(SPE)소주거제유생소C,득도총황동세탈액,용초산려비색법진행측정,검측파장510 nm。결과총황동재0~48μg/mL구유량호적선성관계,상관계수위0.9998,상대표준편차(RSD)소우5%,삼개수평적첨가회수솔위95.31%~98.38%。고찰삼충불동총황동화유생소C비례함량적식품(Vc：총황동=1：1,2：1,10：1),발현수착유생소C함량증가,대총황동측정결과적영향월대。인차,대양품진행전처리,가이측득교위준학적총황동함량。결론본방법양품전처리간단、배보、쾌속、준학,괄용우부함유생소C식품중총황동적함량측정。
Objective To establish a quantitative analysis of total flavonoids with high content of ascorbic acid(vitamin C) in food and solve the interference of ascorbic acid on the measurement of total flavonoids by complexation reaction. Methods A solid phase extraction procedure was developed to degrease and remove pigments by Sep-Pak C18, vitamin C was removed with D101 macro-porous resin, total flavonoids were determined by aluminum nitrate colorimetric using detection wavelength at 510 nm. Results The developed method was successfully used to determine total flavonoids in vitamin C rich fruits/plant extracts. It showed a good linearity in the range of 0~48 μg/mL with r=0.9998. The recoveries were 95.31%~98.38% at 3 spiked levels, and the relative standard deviations (RSDs) were less than 5%. Three kinds of food (ascorbic acid:flavonoids=1:1, 2:1, 10:1) were analyzed, which showed that higher ascorbic acid content could affect the testing result. So, it is necessary to remove the ascorbic acid before coloration in rich ascorbic acid products. Conclusion The method is suitable for simple, environmental, and effective determination of total flavonoids in rich ascorbic acid foods.