中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2015年
5期
332-335
,共4页
李媛媛%王玉洁%刘斌%任伟%邵慧莹%苗志敏%李长贵%刘世国
李媛媛%王玉潔%劉斌%任偉%邵慧瑩%苗誌敏%李長貴%劉世國
리원원%왕옥길%류빈%임위%소혜형%묘지민%리장귀%류세국
白细胞介素13%痛风%rs20541
白細胞介素13%痛風%rs20541
백세포개소13%통풍%rs20541
Interleukin-13%Gout%rs20541
目的 探讨青岛地区汉族男性群体的IL-13基因第四外显子区域+2044C/T(rs20541)多态性与原发性痛风遗传易感性的关系.方法 采用TaqMan荧光定量PCR技术,对青岛地区281例男性原发性痛风患者与265名男性对照的+2044C/T位点进行基因分型.采用Hardy-Weinberg检验确认标本的群体代表性,数据分析采用x2检验和t检验.结果 痛风组中IL-13 +2044C/T位点CC,CT和TT基因型分别为82例、146例和53例(29.2％,52.0％和18.9％),对照组分别为92名、127名和48名(34.7％,47.9％和18.1％),2组比较差异无统计学意义(x2=1.788,P＞0.05);2组的等位基因频率C和T间差异也无统计学意义(分别为55.2％,44.8％;58.2％,41.8％;x2=1.057,P＞0.05).结论 尚不能认为青岛地区汉族男性人群中IL-13 +2044C/T位点基因多态性与痛风有关联性.
目的 探討青島地區漢族男性群體的IL-13基因第四外顯子區域+2044C/T(rs20541)多態性與原髮性痛風遺傳易感性的關繫.方法 採用TaqMan熒光定量PCR技術,對青島地區281例男性原髮性痛風患者與265名男性對照的+2044C/T位點進行基因分型.採用Hardy-Weinberg檢驗確認標本的群體代錶性,數據分析採用x2檢驗和t檢驗.結果 痛風組中IL-13 +2044C/T位點CC,CT和TT基因型分彆為82例、146例和53例(29.2％,52.0％和18.9％),對照組分彆為92名、127名和48名(34.7％,47.9％和18.1％),2組比較差異無統計學意義(x2=1.788,P＞0.05);2組的等位基因頻率C和T間差異也無統計學意義(分彆為55.2％,44.8％;58.2％,41.8％;x2=1.057,P＞0.05).結論 尚不能認為青島地區漢族男性人群中IL-13 +2044C/T位點基因多態性與痛風有關聯性.
목적 탐토청도지구한족남성군체적IL-13기인제사외현자구역+2044C/T(rs20541)다태성여원발성통풍유전역감성적관계.방법 채용TaqMan형광정량PCR기술,대청도지구281례남성원발성통풍환자여265명남성대조적+2044C/T위점진행기인분형.채용Hardy-Weinberg검험학인표본적군체대표성,수거분석채용x2검험화t검험.결과 통풍조중IL-13 +2044C/T위점CC,CT화TT기인형분별위82례、146례화53례(29.2％,52.0％화18.9％),대조조분별위92명、127명화48명(34.7％,47.9％화18.1％),2조비교차이무통계학의의(x2=1.788,P＞0.05);2조적등위기인빈솔C화T간차이야무통계학의의(분별위55.2％,44.8％;58.2％,41.8％;x2=1.057,P＞0.05).결론 상불능인위청도지구한족남성인군중IL-13 +2044C/T위점기인다태성여통풍유관련성.
Objective To explore gene polymorphism of the C/T genotype of +2044C/T in the fourth exon of IL-13 gene in males of Qingdao,and thus to investigate the relationship between the gene polymorphism of IL-13 and primary gout.Methods A total of 281 gout patients and 265 controls were enrolled.The possible association between the polymorphism of IL-13 +2044C/T and gout in Qingdao was investigated and genotype frequencies and allelic frequencies were calculated by real-time polymerase chain reaction (RT-PCR) with Taqman probe method.Hardy-Weinberg was used to verify the representativeness of the sample.Comparison between the groups was performed with x2 test and t-test.Results The frequencies of CC,CT and TT genotypes were 82,146 and 53 (29.2％,52.0％ and 18.9％) respectively among gout patients,while they were 92,127 and 48 (34.7％,47.9％ and 18.1％),respectively among the controls.There was no statistical significant difference in IL-13 +2044C/T genotype frequencies between gout patients and controls (x2=1.788,P＞O.05).The allele frequencies of C and T in gout patients were different from those in the controls (55.2％,44.8％;58.2％,41.8％;x2=1.057,P＞0.05).Conclusion Results of the present study suggest that the C/T genotype of +2044C/T in the fourth exon of IL-13 gene is not associated with gout in males of Qingdao.
