CAJ | 학술논문

该文利用酞菁钴纳米粒子修饰氧化石墨烯（NanoCoPc/GO）用于构建无酶的信号放大型电化学免疫传感器来灵敏地检测降钙素原（PCT）。纳米酞菁钴和氧化石墨烯都具有类似于天然过氧化物酶的性质可以催化氧化H2O2。因此，当H2O2的存在时，NanoCoPc/GO通过催化H2O2实现对电活性物质的信号的放大。NanoCoPc/GO作为模拟酶用于电化学放大时，可以避免天然酶的缺点比如价格昂贵和容易随着环境变化而发生变性。结果表明，该免疫传感器检测PCT的线性范围在0.025~5.0 ng/mL，最低检测限为8 pg/mL。
해문이용태정고납미입자수식양화석묵희（NanoCoPc/GO）용우구건무매적신호방대형전화학면역전감기래령민지검측강개소원（PCT）。납미태정고화양화석묵희도구유유사우천연과양화물매적성질가이최화양화H2O2。인차，당H2O2적존재시，NanoCoPc/GO통과최화H2O2실현대전활성물질적신호적방대。NanoCoPc/GO작위모의매용우전화학방대시，가이피면천연매적결점비여개격앙귀화용역수착배경변화이발생변성。결과표명，해면역전감기검측PCT적선성범위재0.025~5.0 ng/mL，최저검측한위8 pg/mL。
In this work, a signal-amplified electrochemical immunosensor based on cobalt phthalocyanine nanoparticles decorated graphene oxide nanocomposite (NanoCoPc/GO) was proposed for sensitive detection procalcitonin (PCT). Here, both cobalt phthalocyanine nanoparticles and graphene oxide exhibited an intrinsic enzyme mimetic activity similar to natural peroxidase. In the present of H2O2, an enhanced electrochemical signal was obtained as the catalysis of NanoCoPc/GO to H2O2. Importantly, NanoCoPc/GO as peroxidase mimetic applied to electrochemical amplification system could avoid the disadvantage of nature enzymes such as expensive and easily denatured by environmental changes. The proposed immunosensor showed good sensitivity for quantitative determination of PCT.