CAJ | 학술논문

蛋白激酶催化蛋白磷酸化是一种非常重要的蛋白质翻译后修饰方式，能够调节细胞内大多数蛋白的功能，在众多生理过程中发挥着至关重要的作用。磷酸化过程的异常以及激酶的过度表达往往与许多疾病密切相关，如癌症、糖尿病和老年痴呆症。因而，测定蛋白激酶的活性及筛选蛋白激酶抑制剂对基础生物医学研究和酶靶药物的开发是非常重要的。近年来，研究工作者们采用电化学法、比色法以及荧光检测法等方法研究了对蛋白激酶活性及抑制性的检测。该文回顾了蛋白激酶活性测定及抑制性检测的研究进展，主要关注了荧光检测法的应用，并对其未来的发展进行展望。
단백격매최화단백린산화시일충비상중요적단백질번역후수식방식，능구조절세포내대다수단백적공능，재음다생리과정중발휘착지관중요적작용。린산화과정적이상이급격매적과도표체왕왕여허다질병밀절상관，여암증、당뇨병화노년치태증。인이，측정단백격매적활성급사선단백격매억제제대기출생물의학연구화매파약물적개발시비상중요적。근년래，연구공작자문채용전화학법、비색법이급형광검측법등방법연구료대단백격매활성급억제성적검측。해문회고료단백격매활성측정급억제성검측적연구진전，주요관주료형광검측법적응용，병대기미래적발전진행전망。
Protein phosphorylation catalyzed by protein kinase is one of the most important post-translational modifications, which adjusts the function of most proteins in cells and plays a significant regulatory role in many vital biological processes. Aberrant protein-phosphorylation states and kinase activity are closely associated with many human diseases, including cancer, diabetes and Alzheimer's disease. Thus, detecting the kinase activity and inhibitor screening are essential for fundamental biochemical research and kinase-targeted drug discovery. Recently, utilizing electrochemical, colorimetric and fluorescence techniques, researchers have developed different methods for monitoring the activity and inhibition of protein kinase. This article reviews the recent advances in the development of assays for protein kinase activity and inhibition, mainly focused assays based on fluorescence methods, and then offers perspectives on future developments.