CAJ | 학술논문

目的：为保健食品中非法添加药物的发现提供筛查策略和方法，以弥补现有检测方法的不足。方法首先针对可以实现或者辅助实现保健食品声称功能的药物建立了对应的信息库，对其主要临床功效和副作用进行了信息汇总。其次通过质谱单离子扫描和子离子扫描，建立上述物质的质谱筛查信息。同时按照不同能量水平，建立不同信号强度的质量信息库。进而作为主体信号，建立液相质谱多反应检测的方法，及其液质联用采集信息，并选择了不同剂型、不同基体的保健食品作为基质，建立了阴性、添加阳性的数据信息库。形成了治疗信息、分析检测信号及确证信息为线索的筛查体系和方式。结果以缓解体力疲劳类药物为例，通过对近百批相关保健食品进行筛查，可见阳性结果发现率较一般方法提高了100%，而对于未知物质的发现提高了300%，验证了以信息依赖型为筛查主线的方法策略。结论信息依赖型保健食品非法添加药物筛查为保健食品中非法添加药物的发现提供了新的途径。
목적：위보건식품중비법첨가약물적발현제공사사책략화방법，이미보현유검측방법적불족。방법수선침대가이실현혹자보조실현보건식품성칭공능적약물건립료대응적신식고，대기주요림상공효화부작용진행료신식회총。기차통과질보단리자소묘화자리자소묘，건립상술물질적질보사사신식。동시안조불동능량수평，건립불동신호강도적질량신식고。진이작위주체신호，건립액상질보다반응검측적방법，급기액질련용채집신식，병선택료불동제형、불동기체적보건식품작위기질，건립료음성、첨가양성적수거신식고。형성료치료신식、분석검측신호급학증신식위선색적사사체계화방식。결과이완해체력피로류약물위례，통과대근백비상관보건식품진행사사，가견양성결과발현솔교일반방법제고료100%，이대우미지물질적발현제고료300%，험증료이신식의뢰형위사사주선적방법책략。결론신식의뢰형보건식품비법첨가약물사사위보건식품중비법첨가약물적발현제공료신적도경。
Objective To provide screening strategy and method for the discovery of illegally added drugs in healthy foods.MethodFirstly, based on the drugs which could realize or help to realize the promised function of health food, the corresponding database was established, which was aimed at collecting main clinical efficacy and side effects of drugs. Secondly, through scanning of mass spectrometry single ion and product ion, the screening information of mass spectrometry was founded. Meanwhile, according to the different energy levels, database based on different signal intensities was set up. Based on the above methods, liquid chromatography-mass spectrometry (LC-MS) multi-reactions monitoring method was applied and LC-MS information was collected. By choosing health food of different dosage forms and substrates, negative data and added positive data were included in the database. Thus, the screening system and method were formed in terms of therapy information, the analysis of test signals and confirmed information. ResultsThe information-dependent method was validated by screening function to relieve physical fatigue drugs as example. According to the screening of hundreds batches of relevant health food, the discovery rate of positive results increased by 100% compared to the general methods and that of unknown substances risen by 300%. ConclusionThe information-dependent strategy for illegally added drug in healthy food provided a novel path for control of the quality of healthy food.