CAJ | 학술논문

目的：建立固相萃取-同位素稀释超高效液相色谱/串联质谱法同时测定动物源性食品中24种β2-受体激动剂残留量的检测方法。方法样品经β-葡萄糖苷酸酶水解,过混合阳离子(MCX)固相萃取柱净化。采用乙腈和含有0.1%甲酸的水为流动相,梯度洗脱,质谱(ESI+)采用多离子检测模式(MRM)对24种β2-受体激动剂残留量进行检测。结果该方法各组分的最低定量限为0.1~0.5μg/kg,在0.1~20.0μg/kg浓度下呈现良好的线性关系,加标回收率为72.5%~109.3%。结论该方法操作简便,准确,快速,灵敏,可同时检测24种β2-受体激动剂的残留量。
목적：건립고상췌취-동위소희석초고효액상색보/천련질보법동시측정동물원성식품중24충β2-수체격동제잔류량적검측방법。방법양품경β-포도당감산매수해,과혼합양리자(MCX)고상췌취주정화。채용을정화함유0.1%갑산적수위류동상,제도세탈,질보(ESI+)채용다리자검측모식(MRM)대24충β2-수체격동제잔류량진행검측。결과해방법각조분적최저정량한위0.1~0.5μg/kg,재0.1~20.0μg/kg농도하정현량호적선성관계,가표회수솔위72.5%~109.3%。결론해방법조작간편,준학,쾌속,령민,가동시검측24충β2-수체격동제적잔류량。
Objective To establish a novel method for simultaneous determination of 24 kinds of β2-agonists in animal derived food by solid phase extraction-isotopes dilution technique and ultra performance liquid chromatography combined with tandem mass spectrometry. Methods Samples were enzymolysised with β-glucuronidase enzyme. The chromatographic concentration and purification were performed with MCX, and the β2-agonists in the extracts were separated on a reversed phase using a gradient elution program of containing 0.1% formic acid as mobile phase. Using mass spectrometry (ESI+) with multiple reactions monitoring (MRM), identification of the major components of 24 kinds of β2-agonists residues was performed. Results This method showed a good linearity in the range of 0.1~20.0 μg/kg for 24 kinds of β2-agonists in animal derived food, the limits of quantitation (LOQ) of this method were 0.1~0.5 μg/kg, and relative standard deviations (RSDs) were 72.5%~109.3%. Conclusion The method is simple, accurate, fast, and sensitive, which is suitable for detecting 24 kinds of β2-agonist residues in animal derived food.