CAJ | 학술논문

目的：为推动免疫胶体金类现场快速检测技术在水产品药物残留监管领域中的应用。方法本研究采用一套“盲样式”免疫胶体金类快速检测产品质量评价方法，对10种市售的用于检测水产品中孔雀石绿残留的国产胶体金快速检测产品的假阳性率、假阴性率、检出率及稳定性等指标进行验证。结果结果显示，有3种产品能100%准确检测出84个鱼糜样品中是否残留孔雀石绿，且2个不同批次产品的检测结果一致；另外7种产品检出假阳性或假阴性样品，且2个不同批次间产品的稳定性有差异。结论将此类产品应用于水产品质量安全监管之前，有必要对其最低检出限、假阳性率、假阴性率、稳定性等参数进行验证，以确保检测结果的科学性和公正性。
목적：위추동면역효체금류현장쾌속검측기술재수산품약물잔류감관영역중적응용。방법본연구채용일투“맹양식”면역효체금류쾌속검측산품질량평개방법，대10충시수적용우검측수산품중공작석록잔류적국산효체금쾌속검측산품적가양성솔、가음성솔、검출솔급은정성등지표진행험증。결과결과현시，유3충산품능100%준학검측출84개어미양품중시부잔류공작석록，차2개불동비차산품적검측결과일치；령외7충산품검출가양성혹가음성양품，차2개불동비차간산품적은정성유차이。결론장차류산품응용우수산품질량안전감관지전，유필요대기최저검출한、가양성솔、가음성솔、은정성등삼수진행험증，이학보검측결과적과학성화공정성。
Objective To promote the application of colloidal gold rapid detection kits in sea food residual regulation. Methods Ten kinds of rapid detection products which were used to detect the residue of malachite green were tested and evaluated by a kind of blind sample testing method, which included false positive rate (FPR), false negative rate (FNR), and the lowest detectable limit (LDL). Results The results showed that only 3 kinds of product could detect the malachite green residues in 84 fish samples exactly, and there was no difference between the 2 results using different batch of products. The other products detected the false positive/ negative samples, there were different stability in FPR/ FNR of these products when using 2 batches of products. Conculusion The results suggest that it is necessary to evaluate the LDL, FPR and FNR to ensure the impartiality of the test results before application.