CAJ | 학술논문

目的：对2011-15-3等4个茶树新品系进行亲缘关系分析,以期为新品系的识别、新品种登记及育种亲本选配提供可靠依据。方法利用文献开发的24对EST-SSR引物,对4个茶树新品系等7份材料进行PCR扩增,在所得相似系数的基础上进行UPGMA聚类,并绘制亲缘关系树状聚类图。结果24对引物共扩增出69个等位位点,平均2.88；平均杂合度观测值(Ho)、平均杂合度期望值(He)、Shannon指数的平均值分别为0.42、0.54、0.75,相似系数变幅为0.47~0.89。槠叶齐、碧香早、高芽齐的后代与其母本相似系数值分别高达0.84、0.79、0.79、0.78。根据相似系数矩阵按UPGMA法进行聚类,在相似系数平均值0.66处可将参试的7份材料分为3类：第Ⅰ类为高芽齐和2011-34-1,第Ⅱ类为槠叶齐、2011-21-1和2011-21-3,第Ⅲ类为碧香早和2011-15-3。结论明确了4个茶树新品系与其他品种的亲缘关系及品种间的遗传距离。
목적：대2011-15-3등4개다수신품계진행친연관계분석,이기위신품계적식별、신품충등기급육충친본선배제공가고의거。방법이용문헌개발적24대EST-SSR인물,대4개다수신품계등7빈재료진행PCR확증,재소득상사계수적기출상진행UPGMA취류,병회제친연관계수상취류도。결과24대인물공확증출69개등위위점,평균2.88；평균잡합도관측치(Ho)、평균잡합도기망치(He)、Shannon지수적평균치분별위0.42、0.54、0.75,상사계수변폭위0.47~0.89。저협제、벽향조、고아제적후대여기모본상사계수치분별고체0.84、0.79、0.79、0.78。근거상사계수구진안UPGMA법진행취류,재상사계수평균치0.66처가장삼시적7빈재료분위3류：제Ⅰ류위고아제화2011-34-1,제Ⅱ류위저협제、2011-21-1화2011-21-3,제Ⅲ류위벽향조화2011-15-3。결론명학료4개다수신품계여기타품충적친연관계급품충간적유전거리。
Objective To analyze the genetic relationship of 2011-15-3 and other 3 new strains of tea, so as to offer some information for the new strains identification, new varieties registration and parental selection. Methods The 7 tea accessions were analyzed by the 24 pairs of EST SSR primers derived according to references. Based on EST-SSR data, a dendrogram was constructed by UPGMA method. Results 69 alleles were amplified totally using 24 EST-SSR primers, on average of 2.88. The observed heterozygosity (Ho), the expected heterozygosity (He) and the Shannon index were 0.42, 0.54 and 0.75 on average, respectively. The similarity coefficient varied from 0.47 to 0.89. The similarity coefficient offspring of Zhuyeqi, Bixiangzao and Gaoyaqi and their female parent were as high as 0.84, 0.79, 0.79 and 0.78, respectively. The 7 materials were classified into 3 groups based on the UPGMA method with the similarity coefficient at 0.66: the first group was Gaoyaqi and 2011-34-1, the second was Zhuyeqi, 2011-21-1 and 2011-21-3, and the third was Bixiangzao and 2011-15-3. Conclusion This paper estimated the phylogenetic relationship between 4 new strains of tea and other varieties and revealed the genetic distance among varieties.