CAJ | 학술논문

目的：研究茶多酚等天然抗氧化剂对香烟烟雾致损的 HEK293细胞的保护效能及 JAK2/STAT3代谢调控机制。方法香烟过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种植物提取物后收集烟气，对 HEK293细胞进行 MTT 对比研究及运用 Western blot 技术分析其Bcl-2、Bax、STAT3蛋白表达量变化。结果香烟烟雾可降低HEK293细胞存活率，呈浓度依赖关系；上调 HEK293细胞中 Bcl-2蛋白相对表达量，下调 Bax、STAT3蛋白相对表达量，且各蛋白变化趋势呈浓度依赖效应；在过滤嘴中添加茶多酚、白芦藜醇、维生素C、芦丁、槲皮素、绿原酸、花青素、原花青素8种抗氧化剂明显抑制了香烟烟雾导致 HEK293细胞死亡，结果具有显著性差异(P<0.05)，其中以茶多酚效果最佳。结论8种植物提取物均能对香烟所致HEK293细胞损伤产生一定的保护作用。
목적：연구다다분등천연항양화제대향연연무치손적 HEK293세포적보호효능급 JAK2/STAT3대사조공궤제。방법향연과려취중첨가다다분、백호려순、유생소C、호정、곡피소、록원산、화청소、원화청소8충식물제취물후수집연기，대 HEK293세포진행 MTT 대비연구급운용 Western blot 기술분석기Bcl-2、Bax、STAT3단백표체량변화。결과향연연무가강저HEK293세포존활솔，정농도의뢰관계；상조 HEK293세포중 Bcl-2단백상대표체량，하조 Bax、STAT3단백상대표체량，차각단백변화추세정농도의뢰효응；재과려취중첨가다다분、백호려순、유생소C、호정、곡피소、록원산、화청소、원화청소8충항양화제명현억제료향연연무도치 HEK293세포사망，결과구유현저성차이(P<0.05)，기중이다다분효과최가。결론8충식물제취물균능대향연소치HEK293세포손상산생일정적보호작용。
Objective To investigate the metabolic regulation mechanism of tea polyphenols and other natural antioxidants antagonizing cigarette smoke damaging HEK293 cells and its JAK2/STAT3. Methods Eight kinds of antioxidants, tea polyphenols, resveratrol, vitamin C, rutin, quercetin, chlorogenicacid, anthocyanins and proanthocyanidins, were added to cigarette filters and the smoke was collected. HEK293 cells were studied by MTT trail data and the protein expression Bcl-2, Bax, and STAT3 were analyzed by Western blot technique. Results Cigarette smoke could reduce the HEK293 cell survival rates in a dose-dependent manner. With up-regulate Bcl-2 protein relative expression, down-regulate Bax, and STAT3 protein relative expression levels, each protein expression levels were in a dose-dependent effect for HEK293 cells. Comparing to the control, adding 8 kinds of antioxidants to cigarette filters could inhibit the increase of HEK293 cells death rates caused by cigarette smoke obviously. The results had significant differences (P<0.05), and tea polyphenols was the best. Conclusion Eight kinds of antioxidants can protect HEK293 cell from damage caused by cigarettes smoke.