CAJ | 학술논문

目的：探讨心肌球样细胞（CDCs）在心力衰竭治疗中的应用前景。方法 Wistar雄性大鼠24只，无菌下取大鼠心脏组织通过胶原酶消化，培养取得CDCs，采用体外培养连续传代（P1～P5），镜下观察可见CDCs团形成。采用流式细胞仪分析细胞表型，并连续观察CDCs的自我更新和心肌细胞分化能力，并用免疫组化检测CDCs中的肌钙蛋白（cTnT）的表达。结果CDCs体外呈指数生长并形成细胞团样结构，流式细胞仪检测显示：CD29、CD44、CD90和CD106为阳性，而CD34、CD45为阴性。CDCs表达了心肌细胞特异性的结构蛋白cTnT，并可在体外培养条件下产生自主搏动。结论 CDCs是具有心肌细胞分化潜能及临床应用前景的细胞供体。
목적：탐토심기구양세포（CDCs）재심력쇠갈치료중적응용전경。방법 Wistar웅성대서24지，무균하취대서심장조직통과효원매소화，배양취득CDCs，채용체외배양련속전대（P1～P5），경하관찰가견CDCs단형성。채용류식세포의분석세포표형，병련속관찰CDCs적자아경신화심기세포분화능력，병용면역조화검측CDCs중적기개단백（cTnT）적표체。결과CDCs체외정지수생장병형성세포단양결구，류식세포의검측현시：CD29、CD44、CD90화CD106위양성，이CD34、CD45위음성。CDCs표체료심기세포특이성적결구단백cTnT，병가재체외배양조건하산생자주박동。결론 CDCs시구유심기세포분화잠능급림상응용전경적세포공체。
Objective To investigate the differentiation potential of cardiosphere- derived cel s ( CDCs ) after expan-sion in vitro . Methods CSCs were isolated by enzymatic digestion of cardiac issue from 24 Wistar rats and their im-munological phenotypes were analyzed by using FACS and immunohistological staining . After expansion up to 5th pas-sage, the ability of self- renewal and myogenic potential were analysed. Results The isolated cardiac stem cel s(CSCs) grew exponential y and exhibited a similar morphology to cardiosphere. FACS analysis revealed positive for CD29, CD44, CD90, CD106and negative for CD34, CD45. Smal fractions of CSCs were undergoing myogenic differentiation, evidenced by the expression of cardiac structural proteins (cTnT) and developing spontaneous contraction. Conclusion CDCs retain myogenic potential after in vitro expansion and thus may be used as donor cel s for cardiac plasticity.