CAJ | 학술논문

文章介绍了一种低成本、快速、自动化的离心式微流控生化分析芯片。该芯片通过整合样本前处理和多生化指标检测，采用了多级微流道与虹吸微阀相结合的方式把样本的前处理、样本输送、样本与生化试剂反应等过程集成于一体，并通过比色检测获得各个生化检测项目的结果。实验结果显示芯片比色孔光程的精密度的变异系数在0.08％～0.52％，通过血糖结果的精密度评估的芯片对样本和稀释液定量以及两者混合的精密度变异系数为1.4%，同时使用芯片测定15个生化项目的日间和批内精密度均小于3.5%，这表明该离心式微流控生化分析芯片符合临床检测要求。
문장개소료일충저성본、쾌속、자동화적리심식미류공생화분석심편。해심편통과정합양본전처리화다생화지표검측，채용료다급미류도여홍흡미벌상결합적방식파양본적전처리、양본수송、양본여생화시제반응등과정집성우일체，병통과비색검측획득각개생화검측항목적결과。실험결과현시심편비색공광정적정밀도적변이계수재0.08％～0.52％，통과혈당결과적정밀도평고적심편대양본화희석액정량이급량자혼합적정밀도변이계수위1.4%，동시사용심편측정15개생화항목적일간화비내정밀도균소우3.5%，저표명해리심식미류공생화분석심편부합림상검측요구。
In this paper, a low-cost, rapid, automated centrifugal microfluidic chip for biochemical analysis was presented. The chip integrates sample pre-processing and multi-biochemical items assay. Through multi-level microlfuidic channels and micro-siphon valves, the chip combines the pre-treatment of samples, sample transportation and the reaction processes between sample and biochemical reagent. The results are obtained by colorimetric detection. It is shown that the reproducibility of cuvette pathlength for chip is from 0.08% to 0.52%. The coefifcient of variation (CV) of sample, diluents metering and mixing for chip by the CV of blood glucose results is 1.4%. The CV of day-to-day and within-run precision for 15 biochemical items are less than 3.5%. Therefore, it is concluded that the centrifugal microlfuidic chip for biochemical analysis can satisfy the clinical testing requirements.