CAJ | 학술논문

外周神经缺损相关疾病给神经外科医生和患者带来严重困扰。自体神经移植被认为是治疗神经缺损的最佳疗法，但常常受到供体神经束长度等因素的限制。另外，人们在神经组织工程原理的基础上，提出组织-电极接口的设想，用以克服传统神经接口的缺陷。而该假设也是建立在体外获得足够长的神经束的基础上提出的。因此，我们急需建立体外神经组织培育体系，用来获得足够长的神经束。背根神经节是外周神经系统的发源地，是建立体外外周神经培育体系的理想原材料。文中，将提取 SD大鼠背根神经节，并使用左旋多聚赖氨酸或者右旋多聚赖氨酸修饰过的 Aclar膜作为修饰作为背根神经节的生长基底。通过观察背根神经节的生长发育状况，我们发现经右旋多聚赖氨酸修饰的 Aclar膜更适合用于背根神经节的体外培育。
외주신경결손상관질병급신경외과의생화환자대래엄중곤우。자체신경이식피인위시치료신경결손적최가요법，단상상수도공체신경속장도등인소적한제。령외，인문재신경조직공정원리적기출상，제출조직-전겁접구적설상，용이극복전통신경접구적결함。이해가설야시건립재체외획득족구장적신경속적기출상제출적。인차，아문급수건입체외신경조직배육체계，용래획득족구장적신경속。배근신경절시외주신경계통적발원지，시건입체외외주신경배육체계적이상원재료。문중，장제취 SD대서배근신경절，병사용좌선다취뢰안산혹자우선다취뢰안산수식과적 Aclar막작위수식작위배근신경절적생장기저。통과관찰배근신경절적생장발육상황，아문발현경우선다취뢰안산수식적 Aclar막경괄합용우배근신경절적체외배육。
Peripheral nerve injury and amputation have plagued neurosurgeons and patients. Autologous nerve grafts has been known as the “golden rule” to repair severe lesions but is limited by the length and potentially losing functions originally from the donor nerves. Meanwhile, tissue-electrode interface was proposed to overcome the limitation of the traditional neural interface, but long autologous nerves with original functions are preferred. So ifnding out a substitute material to replace the autologous nerves as donor nerves becomes critical. Dorsal root ganglia (DRG) proved an ideal original material. In this report, we described a method to isolate the DRG explants from the spinal cord of SD rat, and coated the Aclar iflms with poly-D-Lysine (PDL) and poly-L-Lysine (PLL) respectively. Then the strategy of culturing the DRG explants on the coated Aclar iflms was introduced. An experiment was designed to investigate the growth of the DRG explants, speciifcally the DRG axons cultured on the two separately coated -Aclar iflms. The results of the experiment show that PDL has an advantage in coating the Aclar iflm for culturing the DRG explants for nerve tissue growth.