CAJ | 학술논문

光遗传技术具有在毫秒水平上精细调控某种特定细胞亚群活性的优势，对神经回路和某些神经系统疾病的发病机制和干预治疗等基础研究提供了一个崭新的生物工程学工具。文章基于光遗传技术的特点，研发了一套针对光感基因改造过体外培养细胞与离体脑片 LED光刺激系统，实现远程无线控制 LED整列的刺激时间、频率、占空比等参数，并同步检测培养细胞的理化特性。
광유전기술구유재호초수평상정세조공모충특정세포아군활성적우세，대신경회로화모사신경계통질병적발병궤제화간예치료등기출연구제공료일개참신적생물공정학공구。문장기우광유전기술적특점，연발료일투침대광감기인개조과체외배양세포여리체뇌편 LED광자격계통，실현원정무선공제 LED정렬적자격시간、빈솔、점공비등삼수，병동보검측배양세포적이화특성。
Optogenetics technology has the advantage of regulating the activity of speciifc cell population in milliseconds level, which provides a novel bioengineering tools for understanding the mechanism of the neural circuits and the pathogenesis of some neurological diseases and interventions treatment. Based on the characteristics of optogenetics, we developed a set of LED light stimulation system for optogenetics modiifed in vitro cell culture or brain slice. This device can remotely control stimulation parameters of LED arrays such as time, frequency, duty cycle, and simultaneously detect physicochemical properties of cultured cells.