CAJ | 학술논문

建立高效液相色谱法（HPLC）同时测定染发剂中9种苯二胺类化合物和p-氨基苯酚的方法。采用Discovery RP-Am ide C16柱（250mm×4.6mm，5μm，美国Supelco公司）为固定相，25mmol/L磷酸缓冲液体系（pH=6.0，含0.1%的辛烷磺酸钠）和乙腈为流动相，流速1.0ml/min，柱温30℃，进样量20μL，检测波长250nm和280nm。该方法各组分的线性范围为0.500mg/L~400mg/L，相关系数R为0.9992~0.9999，检出限为18mg/Kg~113mg/Kg，定量限为42mg/Kg~373mg/Kg，相对标准偏差RSD＜10%，加标回收率为86%~104%。该检测方法具有简便、快速、准确等特点，基本满足实际样品的分析要求。
건립고효액상색보법（HPLC）동시측정염발제중9충분이알류화합물화p-안기분분적방법。채용Discovery RP-Am ide C16주（250mm×4.6mm，5μm，미국Supelco공사）위고정상，25mmol/L린산완충액체계（pH=6.0，함0.1%적신완광산납）화을정위류동상，류속1.0ml/min，주온30℃，진양량20μL，검측파장250nm화280nm。해방법각조분적선성범위위0.500mg/L~400mg/L，상관계수R위0.9992~0.9999，검출한위18mg/Kg~113mg/Kg，정량한위42mg/Kg~373mg/Kg，상대표준편차RSD＜10%，가표회수솔위86%~104%。해검측방법구유간편、쾌속、준학등특점，기본만족실제양품적분석요구。
A high performance liquid chromatography(HPLC)method was developed for the determination of 10 derivatives of phenylenediamine in hair dyes. Dyes were separated on a Discovery RP-Am ide C16 silica column(250mm × 4.6mm, 5μm,Supelco),the gradient elution of the compounds was performed by HPLC using mobile phase composed of acetonitrile,phosphate buffer and the ion-pairing reagent with methanol-25mM phosphate buffer(pH=6.0)containing 0.1%1-heptanesulfonic acid sodium salt ,and detection wavelength of 250nm and 280nm.the flow rate was 1.0ml/min and the column temperature was 30℃. The linear range were from 0.500mg/L to 400mg/L and the R were from 0.9992 to 0.9999. the detectable limits were from 18mg/Kg~113mg/Kg and the quantificative limits were from 42mg/Kg to 373mg/Kg ,the coefficient of variations were less than 10% and the recovery rates were from 86% to 104%. The methods of high performance liquid chromatography introduced in this paper is simple,rapid,accurate and is applicable to the analysis of various hair dyes.