CAJ | 학술논문

目的：观察红花黄色素对乳腺癌耐药细胞MCF－7／ADR缝隙连接蛋白Cx43的影响。方法取对数生长期的MCF－7／ADR细胞，以2×105 cells／孔接种于6孔培养板，24 h后分为4组，每组3孔，其中3组分别加入红花黄色素至终浓度25、50、100 mg／L，另一孔设为空白对照组，作用48 h。 Western blot法检测各组MCF－7／ADR细胞中Cx43表达水平。结果不同浓度红花黄色素作用于MCF－7／ADR细胞48 h后， Cx43表达水平均增高，有明显的剂量依赖关系，以50、100 mg／L浓度作用显著（ P＜0．01）。结论红花黄色素能提高乳腺癌耐药细胞MCF－7／ADR缝隙连接蛋白Cx43的表达水平。
목적：관찰홍화황색소대유선암내약세포MCF－7／ADR봉극련접단백Cx43적영향。방법취대수생장기적MCF－7／ADR세포，이2×105 cells／공접충우6공배양판，24 h후분위4조，매조3공，기중3조분별가입홍화황색소지종농도25、50、100 mg／L，령일공설위공백대조조，작용48 h。 Western blot법검측각조MCF－7／ADR세포중Cx43표체수평。결과불동농도홍화황색소작용우MCF－7／ADR세포48 h후， Cx43표체수평균증고，유명현적제량의뢰관계，이50、100 mg／L농도작용현저（ P＜0．01）。결론홍화황색소능제고유선암내약세포MCF－7／ADR봉극련접단백Cx43적표체수평。
Objective To observe the effects of Safflower yellow on gap junction protein Cx 43 in drug resist-ant breast cancer cell line MCF -7/ADR.Methods MCF-7/ADR cells of logarithmic growth phase were inocula-ted in six-well culture dishes (2 ×105 cells each hole),and divided into 4 groups after 24 hours,3 holes in each group.3 groups were added Safflower yellow to the final concentration of 25,50,100 mg/L,respectively.The last group was blank control group.48 h after cultivating,the expression level of Cx43 in drug resistant breast cancer cell line MCF-7/ADR were detected by Western -blot.Results 48 h after different concentration of Safflower yellow acted on MCF-7/ADR cells,the expression level of Cx 43 was increased in a dose -dependent relationship and there were remarkable effects on the concentration of 50,100 mg/L (P<0.01).Conclusion Safflower yellow can improve the expression level of gap junction protein Cx 43 in drug resistant breast cancer cell line MCF -7/ADR.