CAJ | 학술논문

目的：观察虎杖提取物白藜芦醇（ Rev）对放射诱导小鼠肺损伤的保护作用及其防护机制。方法将30只C57 BL／6雄性小鼠实验小鼠随机分为对照组、照射组和照射给药组，每组各10只。照射组和照射给药组小鼠用1％戊巴比妥钠（30 mg／kg）腹腔注射麻醉后固定于照射板架上，每日接受1次137 Cs源γ射线胸部照射，共照射5次。对照组小鼠接受伪照射。照射给药组照射期间及照射后2周予Rev 40 mg／kg每日灌胃给药，对照组和照射组予等量4．8％乙醇载体灌胃。末次照射后15 d，全身麻醉，处死小鼠。观察3组小鼠肺组织病理改变，检测丙二醇（MDA）含量，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX1）活性和基因表达水平。结果与照射组比较，照射给药组在小鼠肺泡炎水平明显改善，肺组织MDA含量显著减少，SOD及GPX1酶活性升高，SOD2及GPX1基因表达水平升高（ P ＜0．05）。结论虎杖提取物Rev可以提高放射性肺损伤小鼠肺组织的氧化应激水平，改善肺组织炎症。
목적：관찰호장제취물백려호순（ Rev）대방사유도소서폐손상적보호작용급기방호궤제。방법장30지C57 BL／6웅성소서실험소서수궤분위대조조、조사조화조사급약조，매조각10지。조사조화조사급약조소서용1％무파비타납（30 mg／kg）복강주사마취후고정우조사판가상，매일접수1차137 Cs원γ사선흉부조사，공조사5차。대조조소서접수위조사。조사급약조조사기간급조사후2주여Rev 40 mg／kg매일관위급약，대조조화조사조여등량4．8％을순재체관위。말차조사후15 d，전신마취，처사소서。관찰3조소서폐조직병리개변，검측병이순（MDA）함량，초양화물기화매（SOD）화곡광감태과양화물매（GPX1）활성화기인표체수평。결과여조사조비교，조사급약조재소서폐포염수평명현개선，폐조직MDA함량현저감소，SOD급GPX1매활성승고，SOD2급GPX1기인표체수평승고（ P ＜0．05）。결론호장제취물Rev가이제고방사성폐손상소서폐조직적양화응격수평，개선폐조직염증。
Objective To observe the protective effects and protective mechanism of resveratrol (Rev) ex-tracted from Polygonum cuspidatum on radiation induced lung injury in mice .Methods 30 C57BL/6 male laborato-ry mice were randomly divided into control group , irradiation group and irradiation drug administration group , 10 mice of each group .Mice in irradiation group and irradiation drug administration group received peritoneal injection of 1%pentobarbital sodium (30 mg/kg), and then were fastened to irradiation grillage .Mice in irradiation group and irra-diation drug administration group received thoracic irradiation of 137 Cs source gamma rays once daily , 5 times totally. Mice in control group received pseudo irradiation .Mice in irradiation drug administration group received daily intra-gastric administration of Rev (40 mg/kg) during irradiation and 2 weeks after irradiation.Mice in other two groups received daily intragastric administration of carrier of 4.8%ethanol at the same dose .Mice in all groups were sacri-ficed 15 days after the final irradiation .The pathological changes of lung tissue , the content of propanediol ( MDA) , the enzyme activities of Chaoyang tiger dismutase ( SOD) and glutathione peroxidase ( GPX1 ) and the expression of gene in mice were observed in 3 groups .Results The level of pulmonary alveolitis in irradiation drug administration group had obviously improved as compared with that in irradiation group , and the content of MDA in lung tissue was decreased , the activities of SOD and GPX 1 were increased , the gene expressions of SOD 2 and GPX1 were increased ( P <0.05).Conclusion Resveratrol extracted from Polygonum cuspidatum can increase the level of oxidative stress in lung tissue of irradiating mice , and improve the infection reaction of lung tissue .