CAJ | 학술논문

目的：观察母婴早期分离对成年大鼠学习记忆的影响，并探讨其可能的神经生物学机制。方法将出生后7 d的仔鼠24只随机分为3组，即正常对照组、母婴部分分离组和母婴完全分离组，各8只。大鼠出生后81～84 d，用Morris水迷宫观察母婴分离对成年大鼠学习记忆的影响，以尼氏染色显示海马CA1区神经元的损伤情况，并检测海马丙二醛（MDA）和超氧化物歧化酶（SOD）的含量。结果母婴部分分离组大鼠逃避潜伏期（EL）比正常对照组延长（P＜0．05），海马CA1区神经元轻度丢失，穿越平台次数、平台象限游泳时间、海马MDA和SOD含量与正常对照组比较差异无统计学意义（ P＞0．05）；母婴完全分离组大鼠EL较正常对照组延长（P＜0．05），穿越平台次数和平台象限的游泳时间比正常对照组和母婴部分分离组均减少（P＜0．05），海马CA1区神经元损伤较母婴部分分离组加重，MDA含量比正常对照组升高（P＜0．05），SOD活性较正常对照组和母婴部分分离组均降低（P＜0．05）。结论母婴早期分离程度越深，大鼠成年期学习记忆能力降低明显，这可能是大鼠脑内氧自由基过度堆积导致海马CA1区神经元损伤所致。
목적：관찰모영조기분리대성년대서학습기억적영향，병탐토기가능적신경생물학궤제。방법장출생후7 d적자서24지수궤분위3조，즉정상대조조、모영부분분리조화모영완전분리조，각8지。대서출생후81～84 d，용Morris수미궁관찰모영분리대성년대서학습기억적영향，이니씨염색현시해마CA1구신경원적손상정황，병검측해마병이철（MDA）화초양화물기화매（SOD）적함량。결과모영부분분리조대서도피잠복기（EL）비정상대조조연장（P＜0．05），해마CA1구신경원경도주실，천월평태차수、평태상한유영시간、해마MDA화SOD함량여정상대조조비교차이무통계학의의（ P＞0．05）；모영완전분리조대서EL교정상대조조연장（P＜0．05），천월평태차수화평태상한적유영시간비정상대조조화모영부분분리조균감소（P＜0．05），해마CA1구신경원손상교모영부분분리조가중，MDA함량비정상대조조승고（P＜0．05），SOD활성교정상대조조화모영부분분리조균강저（P＜0．05）。결론모영조기분리정도월심，대서성년기학습기억능력강저명현，저가능시대서뇌내양자유기과도퇴적도치해마CA1구신경원손상소치。
Objective To observe the effects of early maternal separation on the learning and memory in rats and investigate the possible neurobiological mechanisms .Methods 24 neonatal rats 7 days after birth were randomly divided into normal control group ,partial maternal separation group ( PMS) and complete maternal separation group ( CMS) ,8 rats in each group .The effect of early maternal separation on the learning and memory in rats at 81 to 84 days after birth was observed by Morris water maze ,the damage of neurons in hippocampus CA 1 area was detected by Nissl staining,and the content of malondialdehyde ( MDA) and superoxide dismutase ( SOD) in hippocampus were detected.Results The escape latent period of PMS rats was longer than that in normal control group (P<0.05). There were no statistical significance between PMS group and normal control group on times of crossing platform , swimming time in the platform quadrant and contents of MDA and SOD in hippocampus (P>0.05).There was nean-on mild loss in the hippocampus CA 1 area.The escape latent period in CMS group was longer than that in normal con-trol group (P<0.05),the times of crossing platform and the swimming time in the platform area were reduced as compared with those in normal control group and PMS group (P<0.05),the damage of neurons in CA1 area was ag-gravated as compared with that in PMS group , the content of MDA was higher than that in normal control group ( P<0.05) ,the activity of SOD was decreased as compared with that in normal control group and PMS group (P<0.05).Conclusion The deeper early ma-ternal separation ,the more learning and memory reduc-ing in adult rats,which may be result from the neuron damage of the hippocampus CA 1 area caused by exces-sive accumulation of oxygen free radical in rat brain .