CAJ | 학술논문

目的：研究以益气解毒为主要功效的中药复方对急性辐射损伤小鼠的防护作用。方法设空白组、模型组、氨磷汀组、安多霖组和益气解毒中药复方高、低剂量组，给药14 d。第14天给药后，除空白组外，对其他各组小鼠利用4 Gy 60 Coγ射线进行全身一次性照射。分别于照射后第3、7、14天检测小鼠外周血白细胞数，胸腺指数，脾脏指数，脾脏病理切片，外周血T淋巴细胞CD4＋／CD8＋比值，肝脏超氧化物歧化酶（ SOD）活力和丙二醛（ MDA）含量。结果γ射线全身照射后，模型组小鼠外周血白细胞数显著降低，免疫脏器指数显著下降，脾脏有明显的病理改变，CD4＋／CD8＋比值显著升高，SOD活力显著下降，MDA含量显著升高，机体免疫力下降；于照后7 d，SOD活力和MDA含量自行恢复至正常水平；于照后14 d脾脏指数恢复至正常水平。与模型组比较，给药组脾脏病理改变较轻；照后3 d，低剂量组小鼠肝SOD活力显著增强（P＜0．01），MDA含量显著降低（P＜0．05）；照后7 d，低剂量组和高剂量组小鼠脾脏指数显著提高（P＜0．05），低剂量组外周血T淋巴细胞CD4＋／CD8＋比值显著降低（P＜0．05）；照后14 d，低剂量组小鼠胸腺指数显著提高（P＜0．05）。结论低剂量的益气解毒中药复方对γ射线所致的小鼠急性辐射损伤具有一定的保护作用。
목적：연구이익기해독위주요공효적중약복방대급성복사손상소서적방호작용。방법설공백조、모형조、안린정조、안다림조화익기해독중약복방고、저제량조，급약14 d。제14천급약후，제공백조외，대기타각조소서이용4 Gy 60 Coγ사선진행전신일차성조사。분별우조사후제3、7、14천검측소서외주혈백세포수，흉선지수，비장지수，비장병리절편，외주혈T림파세포CD4＋／CD8＋비치，간장초양화물기화매（ SOD）활력화병이철（ MDA）함량。결과γ사선전신조사후，모형조소서외주혈백세포수현저강저，면역장기지수현저하강，비장유명현적병리개변，CD4＋／CD8＋비치현저승고，SOD활력현저하강，MDA함량현저승고，궤체면역력하강；우조후7 d，SOD활력화MDA함량자행회복지정상수평；우조후14 d비장지수회복지정상수평。여모형조비교，급약조비장병리개변교경；조후3 d，저제량조소서간SOD활력현저증강（P＜0．01），MDA함량현저강저（P＜0．05）；조후7 d，저제량조화고제량조소서비장지수현저제고（P＜0．05），저제량조외주혈T림파세포CD4＋／CD8＋비치현저강저（P＜0．05）；조후14 d，저제량조소서흉선지수현저제고（P＜0．05）。결론저제량적익기해독중약복방대γ사선소치적소서급성복사손상구유일정적보호작용。
Objective Qi-tonifying toxin-removing Chinese medicinals can tonify qi and remove toxin. This research studied its protective effects against acute radiation injury in mice.Methods There were six groups, including blank group, model group, high dose group of qi-tonifying toxin-removing Chinese medicinals, low dose group of qi-tonifying toxin-removing Chinese medicinals, amifostine group, and anduolin group.All of the above groups were administered for 14 days.At day 14 after drug administra-tion, all groups of mice, except the blank group, were given 4 Gy 60 Co gamma ray for total body irradia-tion to induce acute radiation injury.At day 3,7, and 14 after irradiation, the mice’ s white blood cell count, thymus index, spleen index, spleen pathological section, the ratio of CD4 +/CD8 +T lymphocyte of peripheral blood, SOD activity and MDA content of liver were examined respectively.Results After gamma ray irradiation in the model group, a significant reduction in white blood cell count were detected. Immune organ index of model group mice decreased significantly.There were also significant pathological changes in the spleen, and marked increase of CD4 +/CD8 +ratio, indicating impaired immunity.SOD activity decreased while MDA content increased significantly.At day 7 after irradiation, SOD activity and MDA content returned to normal level; At day 14 after irradiation, the spleen index returned to normal level.Compared with the model group, the spleen pathological changes of treatment groups were less severe.At day 3 after irradiation, the activity of SOD in liver of low dose group was significantly enhanced (p<0.01);hepatic MDA content significantly decreased (p<0.05).At day 7 after irradia-tion, the spleen index in low dose group and high dose group mice significantly increased (p<0.05), and the ratio of CD4 +/CD8 +T lymphocyte in peripheral blood of low dose group significantly reduced ( p<0.05 ); At day 14 after irradiation, thymus index of low dose group significantly increased ( p <0.05 ) .Conclusion Low dose of formula has protective effects against sublethal doses of gamma ray induced acute radiation injury in mice.