CAJ | 학술논문

目的：观察天敌暴露饮水冲突模型大鼠海马、杏仁核相关脑区的形态结构变化以及疏肝清热健脾中药丹栀逍遥散对其的干预作用。方法经Open－Field 行为学评分后，将雄性Wistar大鼠随机分为正常组、模型组、中药组、西药组，每组8只。模型组、中药组、西药组采用天敌暴露、饮水冲突应激方法建立焦虑模型。于第15天开始，正常组、模型组予蒸馏水灌胃，西药组予盐酸丁螺环酮2 mg／（ kg? d）灌胃，中药组予丹栀逍遥散10．5 g／（ kg? d）灌胃，各组连续给药7 d。光镜下观察海马、杏仁核形态结构变化。结果正常组海马锥体细胞及杏仁核神经细胞核大而圆，细胞之间连接紧密，排列整齐。模型组海马、杏仁核可见大量的神经元出现细胞核染色质向核膜下边聚集，核固缩，形态不规则，细胞之间排列紊乱疏松无规则，细胞周围存在空隙，尤其以海马的CA2和CA3区明显。中药组和西药组较模型组有明显的改善，细胞排列尚紧凑整齐。结论疏肝清热健脾法代表方丹栀逍遥散对焦虑模型大鼠相关脑组织损伤具有一定的改善作用，可促进其损伤的恢复。
목적：관찰천활폭로음수충돌모형대서해마、행인핵상관뇌구적형태결구변화이급소간청열건비중약단치소요산대기적간예작용。방법경Open－Field 행위학평분후，장웅성Wistar대서수궤분위정상조、모형조、중약조、서약조，매조8지。모형조、중약조、서약조채용천활폭로、음수충돌응격방법건립초필모형。우제15천개시，정상조、모형조여증류수관위，서약조여염산정라배동2 mg／（ kg? d）관위，중약조여단치소요산10．5 g／（ kg? d）관위，각조련속급약7 d。광경하관찰해마、행인핵형태결구변화。결과정상조해마추체세포급행인핵신경세포핵대이원，세포지간련접긴밀，배렬정제。모형조해마、행인핵가견대량적신경원출현세포핵염색질향핵막하변취집，핵고축，형태불규칙，세포지간배렬문란소송무규칙，세포주위존재공극，우기이해마적CA2화CA3구명현。중약조화서약조교모형조유명현적개선，세포배렬상긴주정제。결론소간청열건비법대표방단치소요산대초필모형대서상관뇌조직손상구유일정적개선작용，가촉진기손상적회복。
Objective To observe the morphological changes in hippocampus and amygdaloid nucleus of rat model exposed to natural enemies and water drinking conflict, when administered with Danzhi Xiaoyao San ( Cortex Moutan and Furctus Gardeniae Free Wanderer Powder ) which has liver-soothing, heat-clearing and spleen-tonifying effects.Methods Evaluated by the open-field test, the male Wistar rats were randomly divided into four groups:normal group, model group, traditional Chinese herbal medicine group, and western medicine group, with 8 rats in each group.Rats in the model group, traditional Chinese herbal medicine group and western medicine group were exposed to natural enemies and water drinking conflict to establish anxiety model.From the 15th day on, rats in the normal group and model group were offered distilled water, and the rats in the western medicine group started on buspirone hydrochloride, while the rats in the traditional Chinese herbal medicine group were given Danzhi Xiaoyao San using intragastric administration.All the above intervention lasted for 7 consecutive days.Light microscope was used to observe the morphological changes in the hippocampus and amygdaloid nucleus. Results Somas of the neurons of the hippocampus and amygdaloid nucleus were relatively large and round in rats of normal group, and their brain cells lined up in an orderly concentrated manner.In the rats of model group, there was karyopyknosis of a large number of neurons, which were hyperchromatic and deformed;the cells were loosely arranged without patterns and there was increased space between the cells.Such feature was particularly prominent in the CA2 and CA3 regions of the hippocampus. Compared with the model group, cells in the traditional Chinese herbal medicine group and western medicine group appeared significantly better and the cell layout was relatively in order.Conclusion The Chinese herbal medicine, Danzhi Xiaoyao San, the key liver-soothing, heat-clearing and spleen-tonifying formula, may repair the encephalic region injury related to the rat’ s Papez circuit and also facilitate the recovery.