CAJ | 학술논문

目的：观察黄芪注射液联合葛根素注射液对糖尿病肾病KKA y 小鼠78 kD－葡萄糖调节蛋白（GRP78）表达的影响，为中医药治疗糖尿病肾病提供实验依据。方法雄性KKAy 小鼠饲养至12周龄时随机分为模型组和黄芪注射液联合葛根素注射液治疗组；同龄雄性C57BL／6J小鼠作为正常对照组。观察小鼠日常状态，并于16周龄处死小鼠，取血清检测空腹血糖、尿素、肌酐、甘油三酯、总胆固醇，并取肾脏观察肾组织病理形态学变化，免疫组织化学法检测小鼠肾组织中GRP78的表达。结果模型组小鼠空腹血糖、尿素、肌酐、甘油三酯、总胆固醇较对照组明显升高（P ＜0．01）；治疗组小鼠空腹血糖、尿素、肌酐较模型组降低（ P＜0．05或P＜0．01）。形态学观察可见模型组小鼠肾小管上皮细胞胞浆出现空泡；经治疗后，肾小球、肾小管结构完整，病变不明显。免疫组化显示治疗组GRP78表达较模型组明显减少（ P＜0．01）。结论黄芪注射液联合葛根素注射液可在一定程度上抑制KKAy 小鼠肾组织中GRP78表达，减轻内质网应激，保护2型糖尿病KKAy 小鼠的肾功能。
목적：관찰황기주사액연합갈근소주사액대당뇨병신병KKA y 소서78 kD－포도당조절단백（GRP78）표체적영향，위중의약치료당뇨병신병제공실험의거。방법웅성KKAy 소서사양지12주령시수궤분위모형조화황기주사액연합갈근소주사액치료조；동령웅성C57BL／6J소서작위정상대조조。관찰소서일상상태，병우16주령처사소서，취혈청검측공복혈당、뇨소、기항、감유삼지、총담고순，병취신장관찰신조직병리형태학변화，면역조직화학법검측소서신조직중GRP78적표체。결과모형조소서공복혈당、뇨소、기항、감유삼지、총담고순교대조조명현승고（P ＜0．01）；치료조소서공복혈당、뇨소、기항교모형조강저（ P＜0．05혹P＜0．01）。형태학관찰가견모형조소서신소관상피세포포장출현공포；경치료후，신소구、신소관결구완정，병변불명현。면역조화현시치료조GRP78표체교모형조명현감소（ P＜0．01）。결론황기주사액연합갈근소주사액가재일정정도상억제KKAy 소서신조직중GRP78표체，감경내질망응격，보호2형당뇨병KKAy 소서적신공능。
Objective To observe the effects of astragalus and puerarin injections on the expression of 78-kD glucose-regulated protein (GRP78) in KKAy mice with diabetic nephropathy.Methods Male KKAy mice were randomly divided into model group and treatment group, and C57BL/6J mice were used as normal control group.The mice in treatment group were given intraperitoneal astragalus injection and puerarin injection from the 12th week.The mice were sacrificed in the 16th week.The blood serum was collected for detecting the levels of fasting blood glucose, urea, creatinine, triglycerides, and total cholesterol.The kidneys were removed and the renal pathological changes were observed.The expression of GRP78 in the renal tissues was examined using immunohistochemical staining.Results Blood biochemical tests in mice showed that, compared with normal mice, the levels of fasting blood glucose, urea, creatinine, total cholesterol and triglycerides increased significantly in model group (P<0.01). Compared with model group, the levels of fasting blood glucose, urea and the creatinine decreased markedly in treatment group (P<0.05 or P<0.01).Under morphological observation, vacuoles were discovered in the cytoplasm of renal tubular epithelial cells in model group.After treatment, the structure of glomerular and tubular were intact, and there were no apparent pathological changes. Immunohistochemistry showed that the expression of GRP78 protein in treatment group was significantly lower than model group ( P <0.01 ) .Conclusion Astragalus injection and puerarin injection used together can reduce the degree of endoplasmic reticulum stress and protect the function of kidney through inhibiting the expression of GRP78 to certain extent.