CAJ | 학술논문

目的探讨溶质载体家族30/锌转运体,成员8(SLC30A8)基因(rs13266634和rs3802177)和蛋白酪氨酸磷酸酶受体D(PTPRD)基因(rs17584499)单核苷酸多态性与南京地区中老年人群2型糖尿病的相关性.方法对南京地区40岁以上人群行75 g口服葡萄糖耐量试验及问卷调查,选取2型糖尿病1 758例(糖尿病组),正常对照1 970名(对照组),提取外周血基因组DNA,对其SLC30A8和PTPRD基因3个目标位点的基因型进行检测,分析与2型糖尿病的关系.结果 SLC30A8基因位点rs13266634在糖尿病组的基因型频率(CC、CT和TT)分别为35.9％、48.2％和15.9％,对照组为33.3％、48.8％和17.9％,糖尿病组C等位基因频率高于对照组(x2=3.986,P=0.046),C等位基因携带者患2型糖尿病的风险是T等位基因的1.158倍[优势比(OR)=1.158,P=0.005].位点rs3802177在糖尿病组的基因型频率(CC、CT和TT)分别为35.3％、48.2％和16.5％,对照组为32.1％、49.9％和18.0％,糖尿病组C等位基因频率高于对照组(x2=4.085,P =0.043),C等位基因携带者患2型糖尿病的风险是T等位基因的1.162倍(OR =1.162,P=0.004).PTPRD基因位点rs17584499在糖尿病组的基因型频率(CC、CT和TT)分别为81.9％、16.9％和1.2％,对照组为82.4％、16.5％和1.1％,两组基因频率分布无统计学差异(x2=0.274,P=0.600).结论 SLC30A8基因位点rs13266634和rs3802177的C等位基因可能是2型糖尿病的风险基因,与南京地区中老年人群2型糖尿病相关,未发现PTPRD基因(rs17584499)与本地区2型糖尿病相关.
목적 탐토용질재체가족30/자전운체,성원8(SLC30A8)기인(rs13266634화rs3802177)화단백락안산린산매수체D(PTPRD)기인(rs17584499)단핵감산다태성여남경지구중노년인군2형당뇨병적상관성.방법 대남경지구40세이상인군행75 g구복포도당내량시험급문권조사,선취2형당뇨병1 758례(당뇨병조),정상대조1 970명(대조조),제취외주혈기인조DNA,대기SLC30A8화PTPRD기인3개목표위점적기인형진행검측,분석여2형당뇨병적관계.결과 SLC30A8기인위점rs13266634재당뇨병조적기인형빈솔(CC、CT화TT)분별위35.9％、48.2％화15.9％,대조조위33.3％、48.8％화17.9％,당뇨병조C등위기인빈솔고우대조조(x2=3.986,P=0.046),C등위기인휴대자환2형당뇨병적풍험시T등위기인적1.158배[우세비(OR)=1.158,P=0.005].위점rs3802177재당뇨병조적기인형빈솔(CC、CT화TT)분별위35.3％、48.2％화16.5％,대조조위32.1％、49.9％화18.0％,당뇨병조C등위기인빈솔고우대조조(x2=4.085,P =0.043),C등위기인휴대자환2형당뇨병적풍험시T등위기인적1.162배(OR =1.162,P=0.004).PTPRD기인위점rs17584499재당뇨병조적기인형빈솔(CC、CT화TT)분별위81.9％、16.9％화1.2％,대조조위82.4％、16.5％화1.1％,량조기인빈솔분포무통계학차이(x2=0.274,P=0.600).결론 SLC30A8기인위점rs13266634화rs3802177적C등위기인가능시2형당뇨병적풍험기인,여남경지구중노년인군2형당뇨병상관,미발현PTPRD기인(rs17584499)여본지구2형당뇨병상관.
Objective To explore the relationship between solute carrier family 30/zinc transporter,member 8(SLC30A8)gene(rs13266634 and rs3802177) and protein tyrosine phosphatase receptor type D (PTPRD) gene (rs 17584499) single nucleotide polymorphisms (SNPs) and type 2 diabetes in middle aged and elderly people in Nanjing area.Methods Genomic DNA were extracted from peripheral blood of 1 758 type 2 diabetic patients (diabetes group) and 1 970 healthy controls (control group) who had received 75 g oral glucose tolerance test and questionnaire survey.All patients were all over 40 years old and came from Nanjing area.Genotypes of three target loci of gene SLC30A8 and PTPRD were detected.Relation between type 2 diabetes and genotype distribution were analyzed.Results The frequencies of the three genotypes CC,CT and TT of SLC30A8 rs13266634 were 35.9％,48.2％ and 15.9％ in diabetes group and 33.3％,48.8％ and 17.9％ in control group,respectively.The C allele frequency in diabetes group was higher than that in control group (x2 =3.986,P =0.046).The risk of type 2 diabetes for C allele carriers was 1.158 times that of T allele [odd ratio(OR)=1.158,P =0.005].The frequencies of three genotypes CC,CT and TT of rs3802177 were 35.3％,48.2％ and 16.5％ in diabetes group and 32.1％,49.9％ and 18.0％ in control group,respectively.The C allele frequency in diabetes group was higher than that in control group (x2=4.085,P=0.043).The risk of type 2 diabetes for C allele carriers was 1.162 times that of T allele (OR =1.162,P =0.004).The distribution of C C,CT and TT of PTPRD rs17584499 in control group(81.9％,16.9％ and 1.2％)and diabetes group (82.4％,165％and 1.1％) was not different (x2=0.274,P =0.600).Conclusions The polymorphisms of rs13266634 and rs3802177 in SLC30A8 are associated with type 2 diabetes in middle aged and elderly people in Nanjing area.The C allele might be a risk allele of type 2 diabetes.There is no relationship between rs17584499 in PTPRD and type 2 diabetes in this area.