中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2015年
5期
334-338
,共5页
郭亚%朱小东%曲颂%李龄%葛莲英%黄诗婷%赵伟%苏芳%李烨
郭亞%硃小東%麯頌%李齡%葛蓮英%黃詩婷%趙偉%囌芳%李燁
곽아%주소동%곡송%리령%갈련영%황시정%조위%소방%리엽
STAT1%RNA干扰%慢病毒载体%放射敏感性%鼻咽癌
STAT1%RNA榦擾%慢病毒載體%放射敏感性%鼻嚥癌
STAT1%RNA간우%만병독재체%방사민감성%비인암
STAT1%RNA interference%Lentiviral vector%Radiosensitivity%Nasopharyngeal carcinoma
目的 研究基因STAT1沉默对人放射抗拒鼻咽癌细胞放射敏感性的影响.方法 慢病毒介导的STAT1基因转染放射抗拒鼻咽癌细胞CNE-2R,荧光定量RT-PCR技术检测沉默效果.MTT法检测转染前后细胞增殖活性,流式细胞技术检测细胞周期及凋亡,克隆形成实验检测转染前后细胞放射敏感性变化.结果 慢病毒转染后放射抗拒鼻咽癌细胞STAT1表达降低(F=429.87,P＜0.05)、细胞生长抑制(F=3.88 ～4.63,P＜0.05)、凋亡率增加(F =38.13,P＜0.05)、放射敏感性增加(F=252.80,P＜0.05)、细胞周期中G0/G1、S和G2/M期未见明显差异(P＞0.05).结论 沉默STAT1基因能增加放射抗拒鼻咽癌细胞CNE-2R的放射敏感性.
目的 研究基因STAT1沉默對人放射抗拒鼻嚥癌細胞放射敏感性的影響.方法 慢病毒介導的STAT1基因轉染放射抗拒鼻嚥癌細胞CNE-2R,熒光定量RT-PCR技術檢測沉默效果.MTT法檢測轉染前後細胞增殖活性,流式細胞技術檢測細胞週期及凋亡,剋隆形成實驗檢測轉染前後細胞放射敏感性變化.結果 慢病毒轉染後放射抗拒鼻嚥癌細胞STAT1錶達降低(F=429.87,P＜0.05)、細胞生長抑製(F=3.88 ～4.63,P＜0.05)、凋亡率增加(F =38.13,P＜0.05)、放射敏感性增加(F=252.80,P＜0.05)、細胞週期中G0/G1、S和G2/M期未見明顯差異(P＞0.05).結論 沉默STAT1基因能增加放射抗拒鼻嚥癌細胞CNE-2R的放射敏感性.
목적 연구기인STAT1침묵대인방사항거비인암세포방사민감성적영향.방법 만병독개도적STAT1기인전염방사항거비인암세포CNE-2R,형광정량RT-PCR기술검측침묵효과.MTT법검측전염전후세포증식활성,류식세포기술검측세포주기급조망,극륭형성실험검측전염전후세포방사민감성변화.결과 만병독전염후방사항거비인암세포STAT1표체강저(F=429.87,P＜0.05)、세포생장억제(F=3.88 ～4.63,P＜0.05)、조망솔증가(F =38.13,P＜0.05)、방사민감성증가(F=252.80,P＜0.05)、세포주기중G0/G1、S화G2/M기미견명현차이(P＞0.05).결론 침묵STAT1기인능증가방사항거비인암세포CNE-2R적방사민감성.
Objective To study the effect of silencing STAT1 on radiation sensitivity of nasopharyngeal carcinoma cells.Methods Lentivirus-mediate STAT1 gene-transfer was performed to radioresistant human nasopharyngeal carcinoma CNE-2R cells and the transfection efficiency was detected by RT-PCR.The cell proliferation was measured by MTT,and the apoptosis was analyzed by flow cytometry.The radiosensitization ratio was measured according to the clone formation efficiency.Results After transfection with STAT1 siRNA,the expression of STAT1 was effectively inhibited in CNE-2R cells (F =429.87,P ＜ 0.05),the cell proliferation was decreased (F =3.88-4.63,P ＜ 0.05),the apoptosis was increased (F =38.13,P ＜ 0.05),the cell radiosensitivity was enhanced (F =252.80,P ＜ 0.05),but the cell cycle distribution at G0/G1,S and G2/M were not influenced (P ＞ 0.05).Conclusions Silencing STAT1 could increase radiosensitization of nasopharyngeal carcinoma cells.
