中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2015年
5期
338-343
,共6页
佘重阳%杨秀芬%顾虹%邓禹%徐军%马凯%刘宁朴
佘重暘%楊秀芬%顧虹%鄧禹%徐軍%馬凱%劉寧樸
사중양%양수분%고홍%산우%서군%마개%류저박
糖尿病视网膜病变%一氧化氮合酶Ⅲ型%内含子%小卫星重复%多态现象,遗传
糖尿病視網膜病變%一氧化氮閤酶Ⅲ型%內含子%小衛星重複%多態現象,遺傳
당뇨병시망막병변%일양화담합매Ⅲ형%내함자%소위성중복%다태현상,유전
Diabetic retinopathy%Nitric oxide synthase type Ⅲ%Introns%Minisatellite repeats%Polymorphism,genetic
目的 探讨内皮型一氧化氮合酶(eNOS)基因内含子的数目可变串连重复多态性(VNTR) 4a/b与糖尿病视网膜病变(DR)的相关性.方法 横断面研究.收集北京市德外社区2型糖尿病患者278例,其中DR组130例,无视网膜病变(DWR)组148例;DR组中增殖性DR(PDR)34例,非增殖性DR(NPDR)96例;收集同一地区非糖尿病志愿者223例作为同源非糖尿病对照人群.采用PCR和琼脂糖凝胶电泳技术检测eNOS基因的VNTR 4a/b基因型.采用R统计软件进行数据分析.采用卡方检验验证基因型分布是否符合Hardy-Weinberg平衡定律.组间年龄比较采用Studentt检验,性别比例、基因型和等位基因出现频率比较采用卡方检验或Fisher精确概率检验,比值比和95%可信区间根据Woolf等式计算.结果 eNOS基因VNTR 4a/b多态性的低频率等位基因a在DR组和DWR组频率分别为10.8%和11.5%,两组相比差异无统计学意义(x2=0.07,P=0.789).4a/b基因型分布DR组(bb 78.5%,ab 21.5%,aa 0.0%)与DWR组(bb 77.7%,ab 21.6%,aa 0.7%)相比差异亦无统计学意义(x2=0.88,P=0.643).该多态性的等位基因及基因型频率在糖尿病组与非糖尿病组之间的差异存在统计学意义(x2=8.75,10.39;P=0.003,0.006),但是在进行年龄、空腹血糖、胆固醇、血脂等因素校正后无意义(x2=0.97,1.25;P=3.224,0.812).在多因素回归分析模型中,纳入患者入组年龄、尿微量白蛋白定量、胰岛素使用、肌酐、糖化血红蛋白及血糖等变量后,VNTR 4a/b多态性与DR发病仍无相关性(OR=0.37,95%可信区间:0.15~0.95).结论 eNOS基因内含子重复序列4a/b多态性与2型糖尿病人群DR的发病无明显相关性.
目的 探討內皮型一氧化氮閤酶(eNOS)基因內含子的數目可變串連重複多態性(VNTR) 4a/b與糖尿病視網膜病變(DR)的相關性.方法 橫斷麵研究.收集北京市德外社區2型糖尿病患者278例,其中DR組130例,無視網膜病變(DWR)組148例;DR組中增殖性DR(PDR)34例,非增殖性DR(NPDR)96例;收集同一地區非糖尿病誌願者223例作為同源非糖尿病對照人群.採用PCR和瓊脂糖凝膠電泳技術檢測eNOS基因的VNTR 4a/b基因型.採用R統計軟件進行數據分析.採用卡方檢驗驗證基因型分佈是否符閤Hardy-Weinberg平衡定律.組間年齡比較採用Studentt檢驗,性彆比例、基因型和等位基因齣現頻率比較採用卡方檢驗或Fisher精確概率檢驗,比值比和95%可信區間根據Woolf等式計算.結果 eNOS基因VNTR 4a/b多態性的低頻率等位基因a在DR組和DWR組頻率分彆為10.8%和11.5%,兩組相比差異無統計學意義(x2=0.07,P=0.789).4a/b基因型分佈DR組(bb 78.5%,ab 21.5%,aa 0.0%)與DWR組(bb 77.7%,ab 21.6%,aa 0.7%)相比差異亦無統計學意義(x2=0.88,P=0.643).該多態性的等位基因及基因型頻率在糖尿病組與非糖尿病組之間的差異存在統計學意義(x2=8.75,10.39;P=0.003,0.006),但是在進行年齡、空腹血糖、膽固醇、血脂等因素校正後無意義(x2=0.97,1.25;P=3.224,0.812).在多因素迴歸分析模型中,納入患者入組年齡、尿微量白蛋白定量、胰島素使用、肌酐、糖化血紅蛋白及血糖等變量後,VNTR 4a/b多態性與DR髮病仍無相關性(OR=0.37,95%可信區間:0.15~0.95).結論 eNOS基因內含子重複序列4a/b多態性與2型糖尿病人群DR的髮病無明顯相關性.
목적 탐토내피형일양화담합매(eNOS)기인내함자적수목가변천련중복다태성(VNTR) 4a/b여당뇨병시망막병변(DR)적상관성.방법 횡단면연구.수집북경시덕외사구2형당뇨병환자278례,기중DR조130례,무시망막병변(DWR)조148례;DR조중증식성DR(PDR)34례,비증식성DR(NPDR)96례;수집동일지구비당뇨병지원자223례작위동원비당뇨병대조인군.채용PCR화경지당응효전영기술검측eNOS기인적VNTR 4a/b기인형.채용R통계연건진행수거분석.채용잡방검험험증기인형분포시부부합Hardy-Weinberg평형정률.조간년령비교채용Studentt검험,성별비례、기인형화등위기인출현빈솔비교채용잡방검험혹Fisher정학개솔검험,비치비화95%가신구간근거Woolf등식계산.결과 eNOS기인VNTR 4a/b다태성적저빈솔등위기인a재DR조화DWR조빈솔분별위10.8%화11.5%,량조상비차이무통계학의의(x2=0.07,P=0.789).4a/b기인형분포DR조(bb 78.5%,ab 21.5%,aa 0.0%)여DWR조(bb 77.7%,ab 21.6%,aa 0.7%)상비차이역무통계학의의(x2=0.88,P=0.643).해다태성적등위기인급기인형빈솔재당뇨병조여비당뇨병조지간적차이존재통계학의의(x2=8.75,10.39;P=0.003,0.006),단시재진행년령、공복혈당、담고순、혈지등인소교정후무의의(x2=0.97,1.25;P=3.224,0.812).재다인소회귀분석모형중,납입환자입조년령、뇨미량백단백정량、이도소사용、기항、당화혈홍단백급혈당등변량후,VNTR 4a/b다태성여DR발병잉무상관성(OR=0.37,95%가신구간:0.15~0.95).결론 eNOS기인내함자중복서렬4a/b다태성여2형당뇨병인군DR적발병무명현상관성.
Objective To investigate the association between the variable number of tandem repeats (VNTR) polymorphism 4a/b in the endothelial nitric oxide synthase (eNOS) gene and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus.Methods cross-sectional study.A total of 278 type 2 diabetes patients were recruited,of whom 130 had DR,and 148 had diabetes without retinopathy (DWR).Of the 130 patients with DR,34 had proliferative DR (PDR) and 96 had non-proliferative DR (NPDR).A number of 223 volunteers without diabetes from the same area were recruited as the control group.PCR and agarose gel electrophoresis methods were adopted to determine the 4a/b polymorphism genotypes of the eNOS gene.Statistical analysis was performed using the R statistical analysis package.Genotype distribution was compared using the x2 test.Numerical data were examined by Student t test.Genotypes and allele frequencies between cases and controls were compared using the x2 test or Fisher's exact test.Odd ratios (OR) and 95% confidence intervals (Co were calculated according to the Woolf's equation.Results The frequencies of minor alleles (a) were 10.8% and 11.5% in the DR and DWR group,respectively.There were no statistical differences between the two groups (x2=0.07,P=0.789).Also there were no statistical differences (x2=0.88;P=0.643) in the distributions of the genotypes between DR group (bb 78.5%,ab 21.5%,aa 0.0%) and DWR group(bb 77.7%,ab 21.6%,aa 0.7%).Statistical differences were found in the frequencies of alleles,and the distributions of genotypes between diabetes group and the control group (x2=8.75,10.39,P=0.003,0.006).However,after adjustment for age,blood pressure,cholesterol concentration,blood-fat and so on,it became insignificant(x2=0.97,1.25,P=3.224,0.812).In the multiple regressions model including clinic factors such as the age of onset of diabetes,urinary albumin,insulin using,creatinine,glycated hemoglobin and fast glucose,no evidence showed that eNOS gene VNTR 4a/b was associated with diabetic retinopathy (OR=0.37,95% CI:0.15-0.95).Conclusion There was no significant association between eNOS gene VNTR 4a/b polymorphism and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus.