中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2015年
5期
351-355
,共5页
宋虎平%朱琦%吴琼%宋赛乐%孙红芬%周晓樑%刘晖
宋虎平%硃琦%吳瓊%宋賽樂%孫紅芬%週曉樑%劉暉
송호평%주기%오경%송새악%손홍분%주효량%류휘
糖尿病视网膜病变%细胞黏附%白细胞%髓系细胞%缺氧诱导因子1,α亚基%RNA,小分子干扰%糖尿病,实验性
糖尿病視網膜病變%細胞黏附%白細胞%髓繫細胞%缺氧誘導因子1,α亞基%RNA,小分子榦擾%糖尿病,實驗性
당뇨병시망막병변%세포점부%백세포%수계세포%결양유도인자1,α아기%RNA,소분자간우%당뇨병,실험성
Diabetic retinopathy%Cell adhesion%Leukocytes%Myeloid Cells%Hypoxia-inducible factor 1,alpha subunit%RNA,Ssmall interfering%Diabetes mellitus,experimental
目的 观察缺氧诱导因子1a特异性小干扰RNA(HIF-1α siRNA)对早期糖尿病大鼠视网膜白细胞黏附及髓样细胞活性的影响.方法 实验研究.构建HIF-1α siRNA表达载体pSUPERH 1-siHIF1α.通过链脲佐菌素腹腔内注射诱导大鼠糖尿病模型.建模成功后2个月,取27只大鼠随机分为糖尿病+磷酸盐缓冲液(PBS)玻璃体腔注射组(B组)、糖尿病+pSUPERH 1-siHIF1α玻璃体腔注射转染组(C组)、糖尿病+ pSUPER空载体玻璃体腔注射组(D组),每组9只大鼠.取9只月龄匹配的正常大鼠为对照组(A组).分别以丫啶橙视网膜白细胞造影和髓过氧化物酶(MPO)酶联免疫吸附试剂盒检测视网膜白细胞黏附数量及髓样细胞活性.四组间均数的差异比较采用单因素方差分析,均数间差异的多重比较采用LSD-t检验.结果 丫啶橙视网膜造影检测结果显示,A、B、C、D组视网膜白细胞黏附数量分别为(47.00±3.60)、(155.33±9.01)、(76.00±9.05)、(142.66±10.26)个,差异有统计学意义(F=116.25,P=0.00),C组视网膜白细胞黏附数量显著低于B组(LSD-t检验,P=0.00,95％可信区间为3.56～ 95.10).4个组大鼠视网膜上清液中MPO的浓度分别为(17.24±1.13)、(31.32±2.53)、(21.35±1.06)、(31.33±1.26) μg/L,差异有统计学意义(F=58.68,P=0.00),C组视网膜中MPO水平显著低于B组(LSD-t检验,P=0.00,95％可信区间为6.92～ 13.01).结论 HIF-1α siRNA对早期糖尿病大鼠视网膜白细胞黏附及髓样细胞活性有一定的调节作用.
目的 觀察缺氧誘導因子1a特異性小榦擾RNA(HIF-1α siRNA)對早期糖尿病大鼠視網膜白細胞黏附及髓樣細胞活性的影響.方法 實驗研究.構建HIF-1α siRNA錶達載體pSUPERH 1-siHIF1α.通過鏈脲佐菌素腹腔內註射誘導大鼠糖尿病模型.建模成功後2箇月,取27隻大鼠隨機分為糖尿病+燐痠鹽緩遲液(PBS)玻璃體腔註射組(B組)、糖尿病+pSUPERH 1-siHIF1α玻璃體腔註射轉染組(C組)、糖尿病+ pSUPER空載體玻璃體腔註射組(D組),每組9隻大鼠.取9隻月齡匹配的正常大鼠為對照組(A組).分彆以丫啶橙視網膜白細胞造影和髓過氧化物酶(MPO)酶聯免疫吸附試劑盒檢測視網膜白細胞黏附數量及髓樣細胞活性.四組間均數的差異比較採用單因素方差分析,均數間差異的多重比較採用LSD-t檢驗.結果 丫啶橙視網膜造影檢測結果顯示,A、B、C、D組視網膜白細胞黏附數量分彆為(47.00±3.60)、(155.33±9.01)、(76.00±9.05)、(142.66±10.26)箇,差異有統計學意義(F=116.25,P=0.00),C組視網膜白細胞黏附數量顯著低于B組(LSD-t檢驗,P=0.00,95％可信區間為3.56～ 95.10).4箇組大鼠視網膜上清液中MPO的濃度分彆為(17.24±1.13)、(31.32±2.53)、(21.35±1.06)、(31.33±1.26) μg/L,差異有統計學意義(F=58.68,P=0.00),C組視網膜中MPO水平顯著低于B組(LSD-t檢驗,P=0.00,95％可信區間為6.92～ 13.01).結論 HIF-1α siRNA對早期糖尿病大鼠視網膜白細胞黏附及髓樣細胞活性有一定的調節作用.
목적 관찰결양유도인자1a특이성소간우RNA(HIF-1α siRNA)대조기당뇨병대서시망막백세포점부급수양세포활성적영향.방법 실험연구.구건HIF-1α siRNA표체재체pSUPERH 1-siHIF1α.통과련뇨좌균소복강내주사유도대서당뇨병모형.건모성공후2개월,취27지대서수궤분위당뇨병+린산염완충액(PBS)파리체강주사조(B조)、당뇨병+pSUPERH 1-siHIF1α파리체강주사전염조(C조)、당뇨병+ pSUPER공재체파리체강주사조(D조),매조9지대서.취9지월령필배적정상대서위대조조(A조).분별이아정등시망막백세포조영화수과양화물매(MPO)매련면역흡부시제합검측시망막백세포점부수량급수양세포활성.사조간균수적차이비교채용단인소방차분석,균수간차이적다중비교채용LSD-t검험.결과 아정등시망막조영검측결과현시,A、B、C、D조시망막백세포점부수량분별위(47.00±3.60)、(155.33±9.01)、(76.00±9.05)、(142.66±10.26)개,차이유통계학의의(F=116.25,P=0.00),C조시망막백세포점부수량현저저우B조(LSD-t검험,P=0.00,95％가신구간위3.56～ 95.10).4개조대서시망막상청액중MPO적농도분별위(17.24±1.13)、(31.32±2.53)、(21.35±1.06)、(31.33±1.26) μg/L,차이유통계학의의(F=58.68,P=0.00),C조시망막중MPO수평현저저우B조(LSD-t검험,P=0.00,95％가신구간위6.92～ 13.01).결론 HIF-1α siRNA대조기당뇨병대서시망막백세포점부급수양세포활성유일정적조절작용.
Objective To investigate the mediation of HIF-1α siRNA to the leukocyte adhesion and myeloid cell's activity in rat's retina at early stage of diabetic retinopathy.Methods Experimental study.HIF-1α specific siRNA expression vector pSUPERH1-siHIF1α was constructed by gene recombination.The rat diabetic model was induced by intraperitoneal injection of streptozotocin.After 2 months of diabetes induction,27 diabetic rats were randomly chosen and assigned to 3 groups,including diabetes and phosphate buffered saline (PBS) vitreous injection group (group B),diabetes and pSUPERH1-siHIF1α vitreous transfect group (group C) and diabetes and pSUPER-retro vitreous transfect group (group D).Each group had 9 rats.Nine age matched health rats were chosen as control group (group A).Retinal leukostasis was quantified with acridine orange leukocyte fluorography.Retinal myeloid cell activity was measured by enzyme linked immunosorbent assay of myeloperoxidase(MPO).The differences of the mean values among the four groups were analyzed by one-factor analysis of variance.The multiple comparisons of the mean values among the four groups were analyzed by LSD-t analysis.Results According to the results of the acridine orange leukocyte fluorography,the numbers of leukocyte adhesion in the four groups were (47.00 ± 3.60),(155.33 ± 9.01),(76.00 ± 9.05),(142.66 ± 10.26),respectively.The differences among them were significant (F=116.25,P=0.00).The number of leukocyte adhesion in the group C was significantly lower than that in group B (LSD-t test,P=0.00,95％CI:3.5~95.10).The levels of retinal MPO in the four groups were (17.24± 1.13),(31.32±2.53),(21.35± 1.06),(31.33± 1.26)μg/L,respectively.The differences among them were significant(F=58.68,P=0.00).The level of retinal MPO in thegroup C was significantly lower than that in group B(LSD-t test,P=0.00,95％ CI:6.92-13.01).Conclusions HIF-lα siRNA may play a role in the mediation of the leukocyte adhesion and myeloid cell's activity in rat's retina at early stage of diabetic retinopathy in vivo.
