中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2015年
5期
356-359
,共4页
抗体,单克隆,人源化%结膜%投药途径%玻璃体内注射%视网膜%渗透
抗體,單剋隆,人源化%結膜%投藥途徑%玻璃體內註射%視網膜%滲透
항체,단극륭,인원화%결막%투약도경%파리체내주사%시망막%삼투
Antibodies,monoclonal,humanized%Conjunctiva%Drug administration routes%Intravitreal injections%Retina%Osmosis
目的 探讨兔眼结膜下注射雷珠单克隆抗体(ranibizumab)是否可以渗透进入视网膜.方法 实验研究.56只新西兰大白兔随机分为4个组:空白对照组(3只)、结膜下注射生理盐水组(3只)、结膜下注射雷珠单克隆抗体组(25只)及玻璃体腔注射雷珠单克隆抗体组(25只),均取右眼进行注射.在注射后1、2、4、8、12周时分别用ELISA和免疫荧光的方法检测兔眼房水中的药物浓度和视网膜中的药物定位情况.药物浓度数据的比较采用t检验.结果 玻璃体腔注射雷珠单克隆抗体组注射后1、2、4、8、12周房水中药物浓度分别为(389.67±22.55)、(319.33±17.93)、(22.67±3.06)、(9.67±1.53)、(7.33±2.18)mg/L.结膜下注射雷珠单克隆抗体组注射后1、2、4、8周房水中药物浓度分别为(53.67±3.21)、(12.67±3.06)、(8.33±1.53)、(2.66±2.08) mg/L,各时间点药物浓度均与玻璃体腔注射组有统计学差异(t=32.96,38.39,7.67,11.07,P＜0.05);12周时房水中未检测到雷珠单克隆抗体.视网膜免疫荧光检查结果与上述结果类似,在结膜下注射组中,只有12周时检测不到雷珠单克隆抗体的阳性信号.结论 雷珠单克隆抗体可以通过结膜下注射的给药方式渗透至视网膜.
目的 探討兔眼結膜下註射雷珠單剋隆抗體(ranibizumab)是否可以滲透進入視網膜.方法 實驗研究.56隻新西蘭大白兔隨機分為4箇組:空白對照組(3隻)、結膜下註射生理鹽水組(3隻)、結膜下註射雷珠單剋隆抗體組(25隻)及玻璃體腔註射雷珠單剋隆抗體組(25隻),均取右眼進行註射.在註射後1、2、4、8、12週時分彆用ELISA和免疫熒光的方法檢測兔眼房水中的藥物濃度和視網膜中的藥物定位情況.藥物濃度數據的比較採用t檢驗.結果 玻璃體腔註射雷珠單剋隆抗體組註射後1、2、4、8、12週房水中藥物濃度分彆為(389.67±22.55)、(319.33±17.93)、(22.67±3.06)、(9.67±1.53)、(7.33±2.18)mg/L.結膜下註射雷珠單剋隆抗體組註射後1、2、4、8週房水中藥物濃度分彆為(53.67±3.21)、(12.67±3.06)、(8.33±1.53)、(2.66±2.08) mg/L,各時間點藥物濃度均與玻璃體腔註射組有統計學差異(t=32.96,38.39,7.67,11.07,P＜0.05);12週時房水中未檢測到雷珠單剋隆抗體.視網膜免疫熒光檢查結果與上述結果類似,在結膜下註射組中,隻有12週時檢測不到雷珠單剋隆抗體的暘性信號.結論 雷珠單剋隆抗體可以通過結膜下註射的給藥方式滲透至視網膜.
목적 탐토토안결막하주사뢰주단극륭항체(ranibizumab)시부가이삼투진입시망막.방법 실험연구.56지신서란대백토수궤분위4개조:공백대조조(3지)、결막하주사생리염수조(3지)、결막하주사뢰주단극륭항체조(25지)급파리체강주사뢰주단극륭항체조(25지),균취우안진행주사.재주사후1、2、4、8、12주시분별용ELISA화면역형광적방법검측토안방수중적약물농도화시망막중적약물정위정황.약물농도수거적비교채용t검험.결과 파리체강주사뢰주단극륭항체조주사후1、2、4、8、12주방수중약물농도분별위(389.67±22.55)、(319.33±17.93)、(22.67±3.06)、(9.67±1.53)、(7.33±2.18)mg/L.결막하주사뢰주단극륭항체조주사후1、2、4、8주방수중약물농도분별위(53.67±3.21)、(12.67±3.06)、(8.33±1.53)、(2.66±2.08) mg/L,각시간점약물농도균여파리체강주사조유통계학차이(t=32.96,38.39,7.67,11.07,P＜0.05);12주시방수중미검측도뢰주단극륭항체.시망막면역형광검사결과여상술결과유사,재결막하주사조중,지유12주시검측불도뢰주단극륭항체적양성신호.결론 뢰주단극륭항체가이통과결막하주사적급약방식삼투지시망막.
Objective To investigate whether ranibizumab can penetrate into the retina after subconjunctival injection in a rabbit model.Methods Experimental study.Fifty-six New Zealand rabbits were randomly divided into four groups:control group (3),saline subconjunctival injection group (3),ranibizumab intravitreal injection group (25) and ranibizumab subconjunctival injection group (25).Each rabbit received single injection in right eye.Ranibizumab's location and concentrations in the retina and aqueous humor were determined by immunofluorescence staining and ELISA at 1,2,4,8,12 weeks after injection.The comparison of ranibizumab concentration data was analyzed by t-test.Results The concentrations of aqueous humor in intravitreal injection group,determined by ELISA,were (389.67±22.55),(319.33± 17.93),(22.67±3.06),(9.67± 1.53) and (7.33±2.18) mg/L at 1,2,4,8,12 weeks respectively.Concentrations of subconjunctival injection group were (53.67±3.21),(12.67±3.06),(8.33±1.53),(2.66±2.08) mg/L at 1,2,4,8 weeks and had a statistical difference compared with intravitreal injection group at each time point(t=32.96,38.39,7.67,11.07,P＜0.05).But there was no detection at 12 weeks.Similar in retina immunofluorescence staining results,the ranibizumab positive signals were detected in all the time points except the last one in subconjunctival injection group.Conclusion Subconjunctival ranibizumab injection may be able to penetrate into retina.
