CAJ | 학술논문

目的：建立HPLC方法测定氯霉素的羊毛脂软膏中氯霉素的含量和有关物质。方法采用高效液相色谱法,色谱柱：安捷伦 C18色谱柱(4．6 mm ×250 mm,5μm),流动相：0．01 mol/L 庚烷磺酸钠缓冲溶液-甲醇(68∶32),检测波长：277 nm,进样量：20μL。结果氯霉素在49．96~199．84μg/mL的浓度范围内与峰面积积分值呈良好的线性关系,精密度、稳定性和重复性试验结果良好,平均回收率为99．12%,RSD为0．51%(n=9),氯霉素与其他杂质的分离度良好。结论该方法具有良好地准确度、精密度、专属性,能良好的控制氯霉素羊毛脂软膏的质量。
목적：건립HPLC방법측정록매소적양모지연고중록매소적함량화유관물질。방법채용고효액상색보법,색보주：안첩륜 C18색보주(4．6 mm ×250 mm,5μm),류동상：0．01 mol/L 경완광산납완충용액-갑순(68∶32),검측파장：277 nm,진양량：20μL。결과록매소재49．96~199．84μg/mL적농도범위내여봉면적적분치정량호적선성관계,정밀도、은정성화중복성시험결과량호,평균회수솔위99．12%,RSD위0．51%(n=9),록매소여기타잡질적분리도량호。결론해방법구유량호지준학도、정밀도、전속성,능량호적공제록매소양모지연고적질량。
Objective To establish an HPLC method for the determination of content and related substances in chloramphenicol and lanolin ointment. Methods The determination was performed on Agilent C18 column (4. 6 mm × 250 mm,5 μm) with the mobile phase of 0. 01 mol/L sodium heptanesulfonate-methanol (68∶32). The detection wavelength was 277 nm and the sample size was 20 μL. Results The linear range of the chloramphenicol was 49. 96~199. 84 μg/mL with an average recovery of 99. 12% (RSD=0. 51%,n=9). The test had good results of pre-cision,stability,repeatability and resolution. Conclusion The method is accurate,precise and specific for the quality control of chloramphenicol and lanolin ointment.