CAJ | 학술논문

目的：建立测定血浆中氧化槐果碱浓度的高效液相色谱法,并用此方法进行苦芪滴丸中氧化槐果碱的药代动力学研究。方法色谱柱为Inertsil-NH2(150 mm ×4．6 mm,5μm)；流动相：乙腈-无水乙醇-3%磷酸水(86∶8∶6)；流速：1．0 mL/min；检测波长：220 nm；进样量：20μL。结果血浆中氧化槐果碱的线性范围：0．20~20μg/mL,r=0．993,最低检测限为0．20μg/mL。日内、日间精密度及准确度、提取回收率符合要求。6只大鼠灌胃给药8．5 mg/kg苦芪滴丸后,血浆中氧化槐果碱Tmax为(4．03±0．72)h,Cmax为(4．00±0．54)ng/mL, t1/2为(0．05±0．66)h,采用梯形法计算,AUC0-t为(30．70±0．54)μg·h/mL,AUC0-∞为(30．8±0．32)ng·h/mL。结论本方法简便、快速、准确、专属性强,可用于苦芪滴丸中氧化槐果碱的药代动力学研究。
목적：건립측정혈장중양화괴과감농도적고효액상색보법,병용차방법진행고기적환중양화괴과감적약대동역학연구。방법색보주위Inertsil-NH2(150 mm ×4．6 mm,5μm)；류동상：을정-무수을순-3%린산수(86∶8∶6)；류속：1．0 mL/min；검측파장：220 nm；진양량：20μL。결과혈장중양화괴과감적선성범위：0．20~20μg/mL,r=0．993,최저검측한위0．20μg/mL。일내、일간정밀도급준학도、제취회수솔부합요구。6지대서관위급약8．5 mg/kg고기적환후,혈장중양화괴과감Tmax위(4．03±0．72)h,Cmax위(4．00±0．54)ng/mL, t1/2위(0．05±0．66)h,채용제형법계산,AUC0-t위(30．70±0．54)μg·h/mL,AUC0-∞위(30．8±0．32)ng·h/mL。결론본방법간편、쾌속、준학、전속성강,가용우고기적환중양화괴과감적약대동역학연구。
Objective To establish an HPLC method for the determination of oxysophocarpin in rat plasma, and study the pharmacokinetics of oxysophocarpin in Kuqi drop pills. Methods The analytical column was Inertsil-NH2 (150 mm × 4. 6 mm,5 μm); mobile phase:acetonitrile-ethanol-3% phosphoric acid water (86∶8∶6);flow rate:1. 0 mL/min; detection wavelength: 220 nm; injection volume: 20 μL. Results The linear concentration range of plasma oxysophocarpin was 0. 20~20 μg/mL,r=0. 993,the lower limits of quantificati were 0. 20 μg/mL. The intra-day and inter-day precision,accuracy,extraction recoveries met the requirement. Six rats were fed by gavage administra-tion of 8. 5 mg/kg Kuqi drop pills,the plasma oxysophocarpin Tmax was (4. 03 ± 0. 72)h,Cmax was (4. 00 ± 0. 54)ng/mL,t1/2 was ( 0. 053 ± 0. 66 ) h, AUC0-t was ( 30. 70 ± 0. 54 ) μg·h/mL, AUC0-∞ was ( 30. 8 ± 0. 32 ) ng·h/mL. Conclusion The method is simple,rapid,accurate,specific,it can be used for the pharmacokinetic study of oxyso-phocarpin in Kuqi drop pills.