CAJ | 학술논문

目的：探讨重组人淀粉样蛋白前体Kunitz型蛋白酶抑制剂结构域变异体( Reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant,rhKD/APPvar)对实验性急性肝损伤小鼠肝细胞增殖能力的影响。方法利用实验鼠的四氯化碳( CCl4)慢性肝损伤模型,采用免疫组化方法,检测各组增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)阳性细胞数。结果正常对照组的 PCNA 阳性细胞数为(2±2)个/视野,模型组的PCNA阳性细胞数为(6±3)个/视野,rhKD/APPvar小、中、大剂量组的PCNA阳性细胞个数分别为(29±110)个/视野、(38±10)个/视野、(55±12)个/视野,抑肽酶组的 PCNA 阳性细胞个数为(24±9)个/视野,rhKD/APP组的PCNA阳性细胞个数为(19±5)个/视野。与模型组相比,rhKD/APPvar各剂量组PCNA阳性细胞数目均显著增加( P<0．05),并且存在剂量依赖关系。 rhKD/APPvar各剂量组阳性细胞数多于抑肽酶组。结论 rhKD/APPvar能够促进急性肝损伤小鼠肝细胞增殖,其作用随着剂量的增加而增强。
목적：탐토중조인정분양단백전체Kunitz형단백매억제제결구역변이체( Reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant,rhKD/APPvar)대실험성급성간손상소서간세포증식능력적영향。방법이용실험서적사록화탄( CCl4)만성간손상모형,채용면역조화방법,검측각조증식세포핵항원(Proliferating cell nuclear antigen,PCNA)양성세포수。결과정상대조조적 PCNA 양성세포수위(2±2)개/시야,모형조적PCNA양성세포수위(6±3)개/시야,rhKD/APPvar소、중、대제량조적PCNA양성세포개수분별위(29±110)개/시야、(38±10)개/시야、(55±12)개/시야,억태매조적 PCNA 양성세포개수위(24±9)개/시야,rhKD/APP조적PCNA양성세포개수위(19±5)개/시야。여모형조상비,rhKD/APPvar각제량조PCNA양성세포수목균현저증가( P<0．05),병차존재제량의뢰관계。 rhKD/APPvar각제량조양성세포수다우억태매조。결론 rhKD/APPvar능구촉진급성간손상소서간세포증식,기작용수착제량적증가이증강。
Objective To study the effect of reorganization human Kunitz protease inhibitor domain of amy-loid protein precursor variant ( rhKD/APPvar) on hepatocyte proliferation ability in mice with experimental acute liver injury and provide theoretical basis for study on protective effect of rhKD/APPvar on acute liver injury. Methods The acute hepatic injury model of rats were induced by carbon tetrachloride (CCl4). Immunohistochemical method was used to observe the proliferating cell nuclear antigen ( PCNA)-positive cell number in various groups. Results The positive cell number in control group was (2 ± 2)/vision;(6 ± 3)/vision in model group;(29 ± 11)/vision,(38 ± 10)/vision and (55 ± 12)/vision in low,middle,and high dose rhKD/APPvar groups; (24 ± 9)/vision in aprotinin group,(19 ± 5) /vision in rhKD/APP group. The positive cell numbers in rhKD/APPvar group markely increased compared with model group(P<0. 05),the positive cell number increased with the increase of rhKD/APPvar. The positive cell num-bers in rhKD/APPvar groups were more than those in aprotinin group. Conclusion The rhKD/APPvar could promote the proliferation of acute injured liver cells and the effect could be enhanced by titrating of rhKD/APPvar.