临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2015年
5期
422-426
,共5页
王秀梅%郭阳%魏圣华%曹潇方%谢晓华
王秀梅%郭暘%魏聖華%曹瀟方%謝曉華
왕수매%곽양%위골화%조소방%사효화
口腔鳞癌%星形细胞上调基因-1( AEG-1)%预后
口腔鱗癌%星形細胞上調基因-1( AEG-1)%預後
구강린암%성형세포상조기인-1( AEG-1)%예후
Oral squamous cell carcinoma%Astrocyte elevated gene-1( AEG-1)%Prognosis
目的：探讨星形细胞上调基因?1（ AEG?1）在口腔鳞状细胞癌中的表达及其与临床病理特征和患者预后的关系。方法应用Western blotting检测AEG?1在口腔鳞癌细胞系（ CAL27、SCC4、Tca8113、Tb、Ts）及人永生化表皮细胞HaCaT中的表达；应用免疫组织化学方法检测AEG?1在87例口腔鳞癌和20例正常口腔黏膜组织中的表达，并分析AEG?1表达与口腔鳞癌临床病理特征的关系；采用Kaplan?Meier法进行生存分析，Cox风险比例回归模型分析影响口腔鳞癌患者预后的独立因素。结果 AEG?1在口腔鳞癌细胞系CAL27、SCC4、Ts及Tb中高表达；AEG?1在口腔鳞癌和正常口腔黏膜组织中的高表达率分别为51?7％（45／87）和10?0％（2／20），差异有统计学意义（ P＝0?001）。口腔鳞癌的AEG?1表达与分化程度、临床分期、T分期及淋巴结转移有关；生存分析显示，AEG?1低表达组的5年生存率为38?7％，高于高表达组的18?0％，差异有统计学意义（ P＝0?001）；单因素生存分析显示AEG?1、临床分期、T分期及淋巴结转移为影响口腔鳞癌预后的因素；Cox多因素分析显示AEG?1是影响口腔鳞癌患者预后的独立因素（ RR＝1?948，95％CI：1?013～3?744，P＝0?046）。结论 AEG?1过表达与口腔鳞癌的发生、发展密切相关，可作为判断口腔鳞癌恶性程度和不良预后的参考指标。
目的：探討星形細胞上調基因?1（ AEG?1）在口腔鱗狀細胞癌中的錶達及其與臨床病理特徵和患者預後的關繫。方法應用Western blotting檢測AEG?1在口腔鱗癌細胞繫（ CAL27、SCC4、Tca8113、Tb、Ts）及人永生化錶皮細胞HaCaT中的錶達；應用免疫組織化學方法檢測AEG?1在87例口腔鱗癌和20例正常口腔黏膜組織中的錶達，併分析AEG?1錶達與口腔鱗癌臨床病理特徵的關繫；採用Kaplan?Meier法進行生存分析，Cox風險比例迴歸模型分析影響口腔鱗癌患者預後的獨立因素。結果 AEG?1在口腔鱗癌細胞繫CAL27、SCC4、Ts及Tb中高錶達；AEG?1在口腔鱗癌和正常口腔黏膜組織中的高錶達率分彆為51?7％（45／87）和10?0％（2／20），差異有統計學意義（ P＝0?001）。口腔鱗癌的AEG?1錶達與分化程度、臨床分期、T分期及淋巴結轉移有關；生存分析顯示，AEG?1低錶達組的5年生存率為38?7％，高于高錶達組的18?0％，差異有統計學意義（ P＝0?001）；單因素生存分析顯示AEG?1、臨床分期、T分期及淋巴結轉移為影響口腔鱗癌預後的因素；Cox多因素分析顯示AEG?1是影響口腔鱗癌患者預後的獨立因素（ RR＝1?948，95％CI：1?013～3?744，P＝0?046）。結論 AEG?1過錶達與口腔鱗癌的髮生、髮展密切相關，可作為判斷口腔鱗癌噁性程度和不良預後的參攷指標。
목적：탐토성형세포상조기인?1（ AEG?1）재구강린상세포암중적표체급기여림상병리특정화환자예후적관계。방법응용Western blotting검측AEG?1재구강린암세포계（ CAL27、SCC4、Tca8113、Tb、Ts）급인영생화표피세포HaCaT중적표체；응용면역조직화학방법검측AEG?1재87례구강린암화20례정상구강점막조직중적표체，병분석AEG?1표체여구강린암림상병리특정적관계；채용Kaplan?Meier법진행생존분석，Cox풍험비례회귀모형분석영향구강린암환자예후적독립인소。결과 AEG?1재구강린암세포계CAL27、SCC4、Ts급Tb중고표체；AEG?1재구강린암화정상구강점막조직중적고표체솔분별위51?7％（45／87）화10?0％（2／20），차이유통계학의의（ P＝0?001）。구강린암적AEG?1표체여분화정도、림상분기、T분기급림파결전이유관；생존분석현시，AEG?1저표체조적5년생존솔위38?7％，고우고표체조적18?0％，차이유통계학의의（ P＝0?001）；단인소생존분석현시AEG?1、림상분기、T분기급림파결전이위영향구강린암예후적인소；Cox다인소분석현시AEG?1시영향구강린암환자예후적독립인소（ RR＝1?948，95％CI：1?013～3?744，P＝0?046）。결론 AEG?1과표체여구강린암적발생、발전밀절상관，가작위판단구강린암악성정도화불량예후적삼고지표。
Objective To detect the expression of astrocyte elevated gene?1 ( AEG?1) in oral squamous cell carcinoma ( OS?CC) , and to investigate the relationship between expression of AEG?1 in OSCC and clinicopathological characteristics, prognosis. Methods The expression of AEG?1 was measured by Western blotting in OSCC cells( CAL27, SCC4, Tca8113, Tb, Ts) and HaCaT cell. At the same time, the expressions of AEG?1 of 87 patients with OSCC and 20 cases of normal oral mucosa were detected by immu?nohistochemistry. The relationship between the expression of AEG?1 in OSCC tissues and clinical pathological characteristics were ana?lyzed. The overall survival of OSCC patients was estimated using the Kaplan?Meier method. The factors affecting the prognosis of OSCC patients were analyzed by Cox proportional hazard regression model. Results AEG?1 was high expressed in OSCC cells ( CAL27, SCC4, Ts and Tb) . The high?level expression rates of AEG?1 was 51?7%( 45/87) and 10?0%( 2/20) , respectively in OSCC and nor?mal oral tissues, in which there existed difference statistically (P=0?001).The expression of AEG?1 was closely correlated with differ?entiation, clinical stage, T stage and lymph node metastasis. Univariate analysis showed AEG?1, clinical stage, T stage and lymph node metastases were prognostic factor of OSCC. Multivariate analysis showed AEG?1 was independent prognostic factors in OSCC( RR=1?948,95%CI:1?013?3?744,P=0?046) . Conclusion The overexpression of AEG?1 might play an important role in the occurrence and development of OSCC, and it can be used as a reference index of the malignant degree and poor prognosis of OSCC.
