CAJ | 학술논문

以冬凌草无菌苗为材料,在冬凌草再生植株时期,添加不同浓度的稀土元素镧和铈,用重量法测定其单株平均鲜重及干重,用高效液相法测定冬凌草甲素、乙素、迷迭香酸的含量,参考农作物、园艺作物种质评比的方法,根据冬凌草生产中各项指标的重要性,设置权重,计算加权后得到综合评分对其进行综合评价,探讨稀土元素镧、铈对冬凌草再生植株产量及次生代谢产物冬凌草甲素、乙素、迷迭香酸含量的影响.结果表明：空白对照组综合评分为62．99分,添加稀土元素铈的条件下,1μmol·L-1 CeCl3·7 H 2 O 评分最高为87．96分,添加稀土元素镧的条件下,5μmol·L-1 LaCl3·6 H 2 O 评分最高为74．44分,这表明 CeCl3·7 H 2 O 对冬凌草再生植株生长及次生代谢产物积累的促进作用优于 LaCl3·6 H 2 O；适宜浓度的镧、铈能促进冬凌草再生植株的生长及次生代谢产物冬凌草甲素、冬凌草乙素、迷迭香酸的合成,而高浓度10μmol·L-1 LaCl3·6 H 2 O 具有抑制的情况.该研究结果将对进一步研究稀土元素对冬凌草再生植株生长的促进作用机制奠定基础,为植物组织培养中如何科学合理地使用稀土元素提供理论依据.
이동릉초무균묘위재료,재동릉초재생식주시기,첨가불동농도적희토원소란화시,용중량법측정기단주평균선중급간중,용고효액상법측정동릉초갑소、을소、미질향산적함량,삼고농작물、완예작물충질평비적방법,근거동릉초생산중각항지표적중요성,설치권중,계산가권후득도종합평분대기진행종합평개,탐토희토원소란、시대동릉초재생식주산량급차생대사산물동릉초갑소、을소、미질향산함량적영향.결과표명：공백대조조종합평분위62．99분,첨가희토원소시적조건하,1μmol·L-1 CeCl3·7 H 2 O 평분최고위87．96분,첨가희토원소란적조건하,5μmol·L-1 LaCl3·6 H 2 O 평분최고위74．44분,저표명 CeCl3·7 H 2 O 대동릉초재생식주생장급차생대사산물적루적촉진작용우우 LaCl3·6 H 2 O；괄의농도적란、시능촉진동릉초재생식주적생장급차생대사산물동릉초갑소、동릉초을소、미질향산적합성,이고농도10μmol·L-1 LaCl3·6 H 2 O 구유억제적정황.해연구결과장대진일보연구희토원소대동릉초재생식주생장적촉진작용궤제전정기출,위식물조직배양중여하과학합리지사용희토원소제공이론의거.
Aseptic seedlings of Rabdosia rubescens were used as the experiment material.In R .rubescens regeneration period,the rare-earth elements La3+ or Ce3+ with different concentrations were added in the culture medium.Fresh and dry weight of the plant were determined by gravimetric method.HPLC method was used for the determination of oridonin,ponicidin and rosmarinic acid content.We determined weight according to evaluating methods of agricultural and horticultural crops and the importance of indexes in the production of R .rubescens .The quality synthetic evalua-tion was also carried out according to weighted mark.The effects of rare earth elements La3+ and Ce3+ on R .rubescens tissue culture and the contents of oridonin,ponicidin,rosmarinic acid in R .rubescens were studied.The synthetic score of blank control group was 61.51 points,under the condition of 1 μmol·L-1 CeCl3 ·7H2 O,the score reached up to the highest value of 87.20 points.But under the condition of 5 μmol·L-1 LaCl3 ·6H2 O,the score 74.44 points.The results of this experiment showed that CeCl3 ·7H2 O had more positive promoting effects on the growth of regeneration plant of R .rubescens and the accumulation of main secondary metabolites than LaCl3 ·6H2 O.The optimal concentration of Ce3+ or La3+ could promote the growth of regeneration plant of R .rubescens and secondary metabolites oridonin,ponicidin and rosmarinic synthesis.However,La3+ concentration in high level inhibited the growth of R .rubescens plant regeneration.The experiment would lay a foundation for further study on the promotion effect on the growth of regeneration plant of R .rubescens .The study of R .rubescens could provide theoretical basis for the rational utilization of rare earth elements.