CAJ | 학술논문

种子萌发是一个较复杂的生理生化过程,是种子贮藏物质在酶的作用下经过一系列反应生成蔗糖、葡萄糖、果糖等各种糖类化合物,为种子萌发提供碳源和能量。该研究利用两个不同来源、籽粒营养成分具有差异的普甜玉米种子动态分析了种子萌发期蔗糖、果糖和葡萄糖代谢及关键水解酶活性的变化。结果表明：在种子萌发过程中,E22和 T26两个普甜玉米种子的物质动员量、物质利用率、蔗糖、葡萄糖和果糖含量均存在遗传差异,其中淀粉含量较高的 T26种子具有较突出的物质利用率,表明淀粉是影响普通甜玉米种子萌发的关键因子；在种子萌发4~8 d、6~10 d 时,E22分别具有较高的蔗糖和葡萄糖含量,而 T26是在萌发10 d时具有较高的果糖含量。随着种子发芽进程,蔗糖合成酶活性、淀粉酶活性都呈逐渐上升的趋势,但淀粉酶活性变幅较明显；进一步关联分析8个种子萌发物质利用性状间关系,结果表明种子萌发期间,种子物质动员量主要受淀粉酶活性影响,而种子物质利用率则主要受糖含量多少制约。因此,提高甜玉米种子萌发期物质利用率对其种子发芽和幼苗生长,增强其与杂草生长的竞争力,提高甜玉米产量均具有重要意义。
충자맹발시일개교복잡적생리생화과정,시충자저장물질재매적작용하경과일계렬반응생성자당、포도당、과당등각충당류화합물,위충자맹발제공탄원화능량。해연구이용량개불동래원、자립영양성분구유차이적보첨옥미충자동태분석료충자맹발기자당、과당화포도당대사급관건수해매활성적변화。결과표명：재충자맹발과정중,E22화 T26량개보첨옥미충자적물질동원량、물질이용솔、자당、포도당화과당함량균존재유전차이,기중정분함량교고적 T26충자구유교돌출적물질이용솔,표명정분시영향보통첨옥미충자맹발적관건인자；재충자맹발4~8 d、6~10 d 시,E22분별구유교고적자당화포도당함량,이 T26시재맹발10 d시구유교고적과당함량。수착충자발아진정,자당합성매활성、정분매활성도정축점상승적추세,단정분매활성변폭교명현；진일보관련분석8개충자맹발물질이용성상간관계,결과표명충자맹발기간,충자물질동원량주요수정분매활성영향,이충자물질이용솔칙주요수당함량다소제약。인차,제고첨옥미충자맹발기물질이용솔대기충자발아화유묘생장,증강기여잡초생장적경쟁력,제고첨옥미산량균구유중요의의。
Seed germination is a complex physiological and biochemical process that the storage material is broken into sucrose,glucose and other sugars compounds under the action of enzyme reaction in order to provide the carbon source and energy for seedling growth.In this study,the dynamics of storage carbohydrate mobilization,sucrose synthase ac-tivity and amylase were analyzed at different seed germination stages by using two different seeds that the grain nutri-ents are different are from different countries in sweet corn.The results showed that there were significant differences for storage carbohydrate mobilization between E22 and T26 including the mobilized seed reserve,seed reserve utiliza-tion,sucrose,glucose and fructose content.Starch is the key factor of seed germination in sweet corn because of the outstanding material utilization in T26.By comparison,the mobilized seed reserve in T26 was lower,while the seed reserve utilization efficiency was higher.During seed germination,sucrose and glucose contents were higher for E22 in 4-8 d and 6-10 d,while fructose content was higher for T26 in 10 d.Amylases and sucrose synthase activities were increased durining the process of seed germination.Grey correlation analysis showed that the mobilized seed reservewas affected by the amylases during seed germination and seed reserve utilization was constrained by sucrose content, glucose content and fructose content.Improving of seed reserve utilization in sweet corn is of great significance to strengthen the seed germination and seedling growth,to enhance its competitiveness with weeds and to increase pro-duction.