临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2015年
10期
788-791
,共4页
加亨%刘振峰%方锐%艾力江·阿斯拉%邓迎杰%宋玉成
加亨%劉振峰%方銳%艾力江·阿斯拉%鄧迎傑%宋玉成
가형%류진봉%방예%애력강·아사랍%산영걸%송옥성
大鼠%骨关节炎%软骨细胞%维药买朱尼%Wnt/ β - catenin 通路
大鼠%骨關節炎%軟骨細胞%維藥買硃尼%Wnt/ β - catenin 通路
대서%골관절염%연골세포%유약매주니%Wnt/ β - catenin 통로
Rats%Osteoarthritis%Cartilage cells%Uygur medicine Maizhuni%Wnt/ β - catenin pathway
目的:研究维药买朱尼对退变软骨细胞中 Wnt/β- catenin 通路的调控作用。方法从1周龄 SD 大鼠关节中分离培养原代软骨细胞,采用人白细胞介素1β诱导软骨细胞退变。取第2代退变软骨细胞,分为对照组、模型组和维药组,采用 CCK -8方法检测细胞增殖状况;采用实时荧光定量 PCR 和免疫印迹法检测 Wnt/β- catenin 通路中 Wnt-3a、β- catenin、GSK -3β的表达水平。结果与对照组相比,模型组中软骨细胞增值速率减慢( P <0.05)。采用维药买朱尼刺激后,软骨细胞的增殖速度显著加快( P <0.05)。Wnt -3a、β- catenin 和 GSK -3β在模型组中表达显著增强( P <0.05),维药买朱尼能降低 Wnt -3a、β- catenin 和 GSK -3β的表达水平( P <0.05)。结论维药买朱尼能通过调节 Wnt/β- catenin 通路来发挥其保护关节软骨细胞的作用。
目的:研究維藥買硃尼對退變軟骨細胞中 Wnt/β- catenin 通路的調控作用。方法從1週齡 SD 大鼠關節中分離培養原代軟骨細胞,採用人白細胞介素1β誘導軟骨細胞退變。取第2代退變軟骨細胞,分為對照組、模型組和維藥組,採用 CCK -8方法檢測細胞增殖狀況;採用實時熒光定量 PCR 和免疫印跡法檢測 Wnt/β- catenin 通路中 Wnt-3a、β- catenin、GSK -3β的錶達水平。結果與對照組相比,模型組中軟骨細胞增值速率減慢( P <0.05)。採用維藥買硃尼刺激後,軟骨細胞的增殖速度顯著加快( P <0.05)。Wnt -3a、β- catenin 和 GSK -3β在模型組中錶達顯著增彊( P <0.05),維藥買硃尼能降低 Wnt -3a、β- catenin 和 GSK -3β的錶達水平( P <0.05)。結論維藥買硃尼能通過調節 Wnt/β- catenin 通路來髮揮其保護關節軟骨細胞的作用。
목적:연구유약매주니대퇴변연골세포중 Wnt/β- catenin 통로적조공작용。방법종1주령 SD 대서관절중분리배양원대연골세포,채용인백세포개소1β유도연골세포퇴변。취제2대퇴변연골세포,분위대조조、모형조화유약조,채용 CCK -8방법검측세포증식상황;채용실시형광정량 PCR 화면역인적법검측 Wnt/β- catenin 통로중 Wnt-3a、β- catenin、GSK -3β적표체수평。결과여대조조상비,모형조중연골세포증치속솔감만( P <0.05)。채용유약매주니자격후,연골세포적증식속도현저가쾌( P <0.05)。Wnt -3a、β- catenin 화 GSK -3β재모형조중표체현저증강( P <0.05),유약매주니능강저 Wnt -3a、β- catenin 화 GSK -3β적표체수평( P <0.05)。결론유약매주니능통과조절 Wnt/β- catenin 통로래발휘기보호관절연골세포적작용。
Objective To study the regulatory function of Uygur medicine Maizhuni on Wnt/ β - catenin pathway in the degeneration process of rat chondrocytes. Methods Primary chondrocytes were isolated from one week old SD rats and treated with interleukin - 1β to induce chondrocyte degeneration. Chondrocytes of passage 2 were randomly divided into control group,model group and Maizhuni group. CCK - 8 assay was performed to detect cell proliferation;quantitative real - time PCR and Western blotting were used to determine the expression of Wnt - 3a,β- catenin and GSK - 3β in the Wnt/ β - catenin pathway. Results Compared to the control group,the proliferation of chondrocytes treated with IL - 1β was obviously decreased( P < 0. 05). Vut Maizhuni administration significantly promoted cell proliferation( P < 0. 05). The expression of Wnt - 3a,β - catenin and GSK - 3β was significantly enhanced in the model group( P < 0. 05). Vut Maizhuni treatment reduced the expres-sion levels of Wnt - 3a,β - catenin and GSK - 3β( P < 0. 05). Conclusion Uygur medicine Maizhuni can protect the chondrocytes against IL- 1β induced degradation by regulation of Wnt/ β - catenin pathway.