临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2015年
10期
785-788
,共4页
黄文志%李倩庆%高宗银
黃文誌%李倩慶%高宗銀
황문지%리천경%고종은
小鼠%视网膜新生血管%视网膜病变%促红细胞生成素产生肝细胞受体%A2%血管内皮生长因子
小鼠%視網膜新生血管%視網膜病變%促紅細胞生成素產生肝細胞受體%A2%血管內皮生長因子
소서%시망막신생혈관%시망막병변%촉홍세포생성소산생간세포수체%A2%혈관내피생장인자
Mouse%Retinal neovascularization%Retinopathy%Erythropoietin producing hepatocellular receptor A2%Vascular endothelial growth factor
目的：探讨促红细胞生成素产生肝细胞受体 A2（EphA2）与血管内皮生长因子（VEGF）在 C57VL/6小鼠氧诱导视网膜新生血管病变模型（OIR）中的表达以及相关性。方法新生 C57VL/6小鼠120只，随机分为实验组和对照组，每组各60只。实验组小鼠建立 OIR 模型，对照组饲养于正常氧环境。各组小鼠于出生后第17天处死，将小鼠眼球行组织切片后 HE 染色，计数突破视网膜内界膜新生血管内皮细胞核数。放射免疫法检测小鼠视网膜组织 EphA2和免疫组织化学染色检测视网膜 VEGF 含量，分析二者的相关性及与视网膜新生血管的关系。结果实验组视网膜大量新生血管形成，实验组小鼠视网膜切片新生血管细胞核计数明显高于对照组（99.10±3.15/个比4.20±0.89/个），差异有统计学意义（ t =28.53，P <0.01）。各组视网膜中 EphA2的 ELISA 检测结果：实验组 EphA2含量明显高于对照组（80.82±20.70 pg/ ml 比14.42±6.80 pg/ ml），差异有统计学意义（ t =23.23，P <0.01）。视网膜免疫组织化学染色显示，VEGF 实验组视网膜组中表达明显升高，在对照组小鼠视网膜有微弱表达，实验组 VEGF 吸光度值高于对照组（5.19±0.75比1.88±0.33），差异有统计学意义（ t =13.42，P <0.05）。EphA2和 VEGF 两者的表达显著相关（ r =0.85，P<0.05）。结论 EphA2与 VEGF 的表达在小鼠视网膜新生血管形成中呈正相关，提示两者可能存在协同效应。
目的：探討促紅細胞生成素產生肝細胞受體 A2（EphA2）與血管內皮生長因子（VEGF）在 C57VL/6小鼠氧誘導視網膜新生血管病變模型（OIR）中的錶達以及相關性。方法新生 C57VL/6小鼠120隻，隨機分為實驗組和對照組，每組各60隻。實驗組小鼠建立 OIR 模型，對照組飼養于正常氧環境。各組小鼠于齣生後第17天處死，將小鼠眼毬行組織切片後 HE 染色，計數突破視網膜內界膜新生血管內皮細胞覈數。放射免疫法檢測小鼠視網膜組織 EphA2和免疫組織化學染色檢測視網膜 VEGF 含量，分析二者的相關性及與視網膜新生血管的關繫。結果實驗組視網膜大量新生血管形成，實驗組小鼠視網膜切片新生血管細胞覈計數明顯高于對照組（99.10±3.15/箇比4.20±0.89/箇），差異有統計學意義（ t =28.53，P <0.01）。各組視網膜中 EphA2的 ELISA 檢測結果：實驗組 EphA2含量明顯高于對照組（80.82±20.70 pg/ ml 比14.42±6.80 pg/ ml），差異有統計學意義（ t =23.23，P <0.01）。視網膜免疫組織化學染色顯示，VEGF 實驗組視網膜組中錶達明顯升高，在對照組小鼠視網膜有微弱錶達，實驗組 VEGF 吸光度值高于對照組（5.19±0.75比1.88±0.33），差異有統計學意義（ t =13.42，P <0.05）。EphA2和 VEGF 兩者的錶達顯著相關（ r =0.85，P<0.05）。結論 EphA2與 VEGF 的錶達在小鼠視網膜新生血管形成中呈正相關，提示兩者可能存在協同效應。
목적：탐토촉홍세포생성소산생간세포수체 A2（EphA2）여혈관내피생장인자（VEGF）재 C57VL/6소서양유도시망막신생혈관병변모형（OIR）중적표체이급상관성。방법신생 C57VL/6소서120지，수궤분위실험조화대조조，매조각60지。실험조소서건립 OIR 모형，대조조사양우정상양배경。각조소서우출생후제17천처사，장소서안구행조직절편후 HE 염색，계수돌파시망막내계막신생혈관내피세포핵수。방사면역법검측소서시망막조직 EphA2화면역조직화학염색검측시망막 VEGF 함량，분석이자적상관성급여시망막신생혈관적관계。결과실험조시망막대량신생혈관형성，실험조소서시망막절편신생혈관세포핵계수명현고우대조조（99.10±3.15/개비4.20±0.89/개），차이유통계학의의（ t =28.53，P <0.01）。각조시망막중 EphA2적 ELISA 검측결과：실험조 EphA2함량명현고우대조조（80.82±20.70 pg/ ml 비14.42±6.80 pg/ ml），차이유통계학의의（ t =23.23，P <0.01）。시망막면역조직화학염색현시，VEGF 실험조시망막조중표체명현승고，재대조조소서시망막유미약표체，실험조 VEGF 흡광도치고우대조조（5.19±0.75비1.88±0.33），차이유통계학의의（ t =13.42，P <0.05）。EphA2화 VEGF 량자적표체현저상관（ r =0.85，P<0.05）。결론 EphA2여 VEGF 적표체재소서시망막신생혈관형성중정정상관，제시량자가능존재협동효응。
Objective To analyze the expression of EphA2 and VEGF in a mouse model of oxygen induced retinopathy and then evaluate their relationship. Methods Totally 120 neonates of C57VL/ 6 mouse were randomly divided into experiment group(n = 60)and control group (n = 60)and OIR models were established in the experiment group. The neonates in the control group were kept in the room air condition. All the mice were sacrificed at P17. The eyes were made into pathological sections for observing and counting the number of vascular cell nuclei on the vit-real side of the internal limiting membrane. And radioimmunoassay was used to compare the EphA2 content of the retina tissue. Immunohistochem-istry analysis of VEGF protein levels in the retina had been done in control mice and the OIR mice. Results The histopathological sections of reti-na showed the number of retinal neovascular endothelial nuclei was 99. 10 ± 3. 15 in the OIR mice and 4. 20 ± 0. 89 in the control group. There was statistically significant difference between those two groups( P < 0. 05). EphA2 radioimmunoassay indicated that the EphA2 content in experi-ment group was 80. 82 ± 20. 70 pg/ ml,but 14. 42 ± 6. 80 pg/ ml in the control group( P < 0. 05). The expression of VEGF in the retina of OIR mice were stronger than the control group( P < 0. 05). There was a positive correlation between the expression of EphA2 and VEGF( P < 0. 05). Conclusion EphA2 and VEGF may interact with each other in the formation of retinal neovascularization.
