CAJ | 학술논문

为了探索低温胁迫下,水杨酸(salicylic acid,SA)提高牡丹叶片耐冷性的生理机制,以8年生牡丹品种‘胡红’20 d 叶龄的叶片为试材,将其浸入0、0．5、1．0、1．5、2．0 mmol·L-1的 SA 水溶液,置于4℃光照培养箱进行暗培养,经0、4、8、12 h 的低温处理后取样测定其活性氧水平和保护酶活性,MDA、可溶性糖和可溶性蛋白含量及细胞膜透性.结果表明：施用低于2．0 mmol·L-1的 SA 可减轻低温对牡丹叶片的伤害程度,降低O －2·、H 2 O 2和 MDA 含量以及细胞膜透性,但能使可溶性糖、可溶性蛋白含量升高,POD、SOD 和 CAT 活性增加.其中1．0 mmol·L-1的 SA 能显著降低叶片组织中 O －2·和 MDA 的含量,明显降低低温引起的叶片水渍化程度,随着低温处理时间的增加,这种效果更加明显.施用2．0 mmol·L-1的 SA 并不能明显减轻低温引起的叶片水渍化程度,当低温处理12 h 后,叶片受到的低温伤害程度和没有施用 SA 时没有明显区别.总之, SA 是否能明显提高牡丹叶片的耐冷性与其浓度有关,1．0 mmol·L-1的 SA 最能提高牡丹叶片的抗寒性,过低和过高浓度的 SA 则不能明显增加叶片的抗寒性.该研究成果能快速应用于牡丹的提早生产,并为增强其它作物的抗寒性提供理论参考.
위료탐색저온협박하,수양산(salicylic acid,SA)제고모단협편내랭성적생리궤제,이8년생모단품충‘호홍’20 d 협령적협편위시재,장기침입0、0．5、1．0、1．5、2．0 mmol·L-1적 SA 수용액,치우4℃광조배양상진행암배양,경0、4、8、12 h 적저온처리후취양측정기활성양수평화보호매활성,MDA、가용성당화가용성단백함량급세포막투성.결과표명：시용저우2．0 mmol·L-1적 SA 가감경저온대모단협편적상해정도,강저O －2·、H 2 O 2화 MDA 함량이급세포막투성,단능사가용성당、가용성단백함량승고,POD、SOD 화 CAT 활성증가.기중1．0 mmol·L-1적 SA 능현저강저협편조직중 O －2·화 MDA 적함량,명현강저저온인기적협편수지화정도,수착저온처리시간적증가,저충효과경가명현.시용2．0 mmol·L-1적 SA 병불능명현감경저온인기적협편수지화정도,당저온처리12 h 후,협편수도적저온상해정도화몰유시용 SA 시몰유명현구별.총지, SA 시부능명현제고모단협편적내랭성여기농도유관,1．0 mmol·L-1적 SA 최능제고모단협편적항한성,과저화과고농도적 SA 칙불능명현증가협편적항한성.해연구성과능쾌속응용우모단적제조생산,병위증강기타작물적항한성제공이론삼고.
In order to explore the physiological mechanism of salicylic acid (SA)improving ability against chilling in-jury of plant leaves,20 d old leaves of Paeonia suffruticosa cultivar ‘huhong’were treated by 0,0.5,1.0,1.5 and 2.0 mmol·L-1 SA under 4 ℃ darkness incubator,and then effects of SA on the contents of H2 O2 ,O-2 ·,soluble sugar, soluble protein and malonaldegyde (MDA)content,the activities of guaiacol-dependent peroxidase (POD),superoxide dismutase (SOD)and catalase (CAT),and membrane permeability in leaves of P .suffruticosa were investigated in 0,4,8,12 h later.The results showed that low concentration of SA(<2.0 mmol·L-1 )could reduce chilling injury of leaves of P .suffruticosa ,the content of H2 O2 ,O-2 · and MDA,and membrane permeability of leaves of P .suffruti-cosa .However,it could increase the contents of soluble sugar and soluble protein,and activities of POD,SOD and CAT of leaves of P .suffruticosa .Especially,1.0 mmol·L-1 SA could decrease contents of O-2 · and MDA,and re-duced chilling injury of leaves of P .suffruticosa .The longer the time of lower temperature treatment was,the stron-ger the protection of SA to leaves of P .suffruticosa was 2 mmol L-1 SA did not significantly reduce the low temperature inducing leaf water salinization degree.The degree of chilling injury of leaves P .suffruticosa un-der 4 ℃ for 12 h whether to 2 mmol L-1 SA use or not was the same.It was concluded that whether SA can ob-viously improve ability against chilling injury of leaves P .suffruticosa depended on SA concentration.1.0 mmol·L-1 SA reduced chilling injury of leaves of P .suffruticosa ,while the lower or higher concentration SA could not obviously increase the cold resistance of leaves of P .suffruticosa .The results can be applied quickly in early spring production of P .suffruticosa ,and provide the theoretical reference for ability against chilling injury of other crops.