CAJ | 학술논문

目的探讨AlloMax补片植入大鼠腹壁不同层面后的组织相容性.方法 SD大鼠随机分为腹壁肌筋膜前组(Onlay组)、肌后组(Sublay组)和腹膜内组(IPOM组),分别在补片植入后1、3、6个月观察补片粘连和皱缩等情况,并对成纤维细胞的长入、支架降解、细胞外基质沉积和血管长入数目进行定量分析.结果大体观察表明Onlay组和Sublay组补片与腹壁整合好于IPOM组,前2组补片在各时间点都显示平展、无皱缩和移位,与腹腔组织和脏器无粘连.6个月时补片大部分或全部与宿主腹壁组织整合.IPOM组则出现网膜与补片粘连,随时间延长而增加,6个月时补片大部分未与腹壁组织整合,并可见补片皱缩和移位.组织学观察:与Onlay组和IPOM组相比,Sublay组在补片植入3个月时,其内出现脂肪细胞.6个月时成纤维细胞和新生血管长入数量也明显少于前2组.另外在各个时间点支架降解和细胞外基质沉积水平也明显低于前二组.结论 Allomax补片植入大鼠腹壁肌筋膜前层的组织相容性优于肌筋膜后层和腹膜内层.
목적 탐토AlloMax보편식입대서복벽불동층면후적조직상용성.방법 SD대서수궤분위복벽기근막전조(Onlay조)、기후조(Sublay조)화복막내조(IPOM조),분별재보편식입후1、3、6개월관찰보편점련화추축등정황,병대성섬유세포적장입、지가강해、세포외기질침적화혈관장입수목진행정량분석.결과 대체관찰표명Onlay조화Sublay조보편여복벽정합호우IPOM조,전2조보편재각시간점도현시평전、무추축화이위,여복강조직화장기무점련.6개월시보편대부분혹전부여숙주복벽조직정합.IPOM조칙출현망막여보편점련,수시간연장이증가,6개월시보편대부분미여복벽조직정합,병가견보편추축화이위.조직학관찰:여Onlay조화IPOM조상비,Sublay조재보편식입3개월시,기내출현지방세포.6개월시성섬유세포화신생혈관장입수량야명현소우전2조.령외재각개시간점지가강해화세포외기질침적수평야명현저우전이조.결론 Allomax보편식입대서복벽기근막전층적조직상용성우우기근막후층화복막내층.
Objective To evaluate biocompatibility of Allomax mesh implanted in different planes of abdominal wall in a rats model.Methods SD rats were randomly assigned to the profacial group (Onlay group),the retro-rectus group (Sublay group) versus the intraperitoneal group(IPOM group) according to different abdominal wall planes the mesh implanted,Adhesion and shrinkage of the mesh were observed,and quantitative measurements were conducted in fibroblast ingrowth,scaffold degradation,extracellular matrix deposition and numbers of vascular ingrowth after 1,3 and 6 months mesh was implanted.Results Macroscopic observation showed both Onlay and Sublay groups was superior to IPOM group in abdominal wall integration,which included shrinkage,relocation and adhesion of the mesh at all the time points,and most or whole of the mesh had incorporated with host abdominal wall at 6 months.Most of the mesh had not incorporated with host abdominal wall and shrinkage and relocation of the mesh were found in IPOM group at 6 months.Microscopic investigation showed lipocytes appeared in the mesh in Sublay group at 3 months,and numbers of ingrowth of fibroblast and neovascularization in Sublay group were significantly less than in Onlay and IPOM group at 6 months.Scaffold degradation and extracellular matrix deposition were remarkably less in Sublay group in comparison with Onlay group and IPOM group after 1,3 and 6 months.Conclusions Biocompatibility of AlloMax mesh implanted in profacial plane of abdominal wall was superior to implanted in retro-rectus plane and intraperitoneal plane as showed in a rat model.