CAJ | 학술논문

目的：探讨宫颈上皮内瘤变（CIN）、宫颈鳞癌组织中组蛋白去乙酰化酶1（HDAC1）的异常表达以及CIN、宫颈鳞癌发病与HPV16/18感染的相关性。方法应用原位杂交（ISH）技术及免疫组织化学（SP）方法检测慢性宫颈炎组织（慢性宫颈炎组）、CIN组织（CINⅠ组、CINⅡ组、CINⅢ组）和宫颈鳞癌组织（宫颈癌组）中HPV16/18感染以及HDAC1的表达。结果慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组中HPV16/18的感染率分别为8.33%、34.78%、48.00%、65.21%、78.00%，组间差异有统计学意义（P<0.05）；HDAC1阳性表达率分别为0、13.04%、32.00%、47.82%和76.00%，组间差异有统计学意义（P<0.05）。HPV16/18感染组的HDAC1阳性表达率高于HPV16/18未感染组（P<0.05）。HDAC1的阳性表达与宫颈癌的临床分期无关（P>0.05），而与组织分化、间质浸润深度及盆腔淋巴结转移相关（P均<0.05）。结论在宫颈癌的发生发展中，HPV16/18感染可能是通过提高组蛋白去乙酰化酶1的水平抑制转录而发挥作用。
목적：탐토궁경상피내류변（CIN）、궁경린암조직중조단백거을선화매1（HDAC1）적이상표체이급CIN、궁경린암발병여HPV16/18감염적상관성。방법응용원위잡교（ISH）기술급면역조직화학（SP）방법검측만성궁경염조직（만성궁경염조）、CIN조직（CINⅠ조、CINⅡ조、CINⅢ조）화궁경린암조직（궁경암조）중HPV16/18감염이급HDAC1적표체。결과만성궁경염조、CINⅠ조、CINⅡ조、CINⅢ조화궁경암조중HPV16/18적감염솔분별위8.33%、34.78%、48.00%、65.21%、78.00%，조간차이유통계학의의（P<0.05）；HDAC1양성표체솔분별위0、13.04%、32.00%、47.82%화76.00%，조간차이유통계학의의（P<0.05）。HPV16/18감염조적HDAC1양성표체솔고우HPV16/18미감염조（P<0.05）。HDAC1적양성표체여궁경암적림상분기무관（P>0.05），이여조직분화、간질침윤심도급분강림파결전이상관（P균<0.05）。결론재궁경암적발생발전중，HPV16/18감염가능시통과제고조단백거을선화매1적수평억제전록이발휘작용。
Objective To explore the anomalous expression of HDAC1 in cervical intraepithelial neoplasia(CIN)and cervical carcinoma and its relationship with the infection of HPV16/18. Methods The expressions of HDAC1 protein and HDAC1 infection were detected by in situ hybridiza?tion(ISH)and immunohistochemistry staining(SP)in tissues from patient with chronic cervicitis(the chronic cervicitis group),CIN(the CINⅠgroup,the CINⅡgroup and the CINⅢgroup)and cervical squamous carcinoma(the cervical carcinoma group). Results The infection rate of HPV16/18 was 8.33%,34.78%,48.00%,65.21%,78.00%in patients with chronic cervicitis,CINⅠ,CINⅡ,CINⅢand cervical carcinoma re?spectively. There was significant difference between groups(P<0.05). The positive expression rate of HDAC1 was 0,13.04%,32.00%,47.82%and 76.00%in chronic cervicitis,CINⅠ,CINⅡ,CINⅢand cervical carcinoma,respectively. The differences were statistically significant(P<0.05). The positive expression of HDAC1 in the HPV16/18 infected patients was significantly higher than those uninfected with HPV16/18(P<0.05). The HDAC1 positive expression had no relationship with clinical stages of cervical carcinoma,however,it was related to the tissue differentia?tion,stromal invasion and pelvic lymph node metastasis(all P<0.05). Conclusion The infection of HPV16/18 may play an important role in the carcinongenesis of SCC through increasing the expression of HDAC1.