中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2015年
5期
456-460
,共5页
肺癌%95D%缺氧%失巢凋亡
肺癌%95D%缺氧%失巢凋亡
폐암%95D%결양%실소조망
lung cancer%95D%hypoxia%anoikis
目的：研究缺氧微环境对人巨细胞肺癌高转移细胞系95D失巢凋亡的影响。方法对95D悬浮培养，建立人肺癌高转移细胞株95D失巢凋亡抵抗细胞模型，实验组进行缺氧培养，对照组正常有氧培养，MTT检测缺氧微环境对于失巢凋亡抵抗95D细胞系生长的影响，流式细胞仪检测2组细胞的凋亡情况，Transwell实验检测2组细胞侵袭能力，Western blot检测缺氧对于失巢凋亡抵抗95D细胞中HIF?1α、VEGF和MMP?2蛋白表达的影响。结果缺氧微环境下失巢凋亡抵抗细胞组生长率为52.9%；凋亡率（40.4%）明显高于非缺氧培养组（21.7%），差异有统计学意义（P<0.05）；侵袭能力降低，穿过膜细胞数（40.1±6.7）明显低于对照组（12.5±7.9），差异有统计学意义（P<0.05）；缺氧微环境下失巢凋亡抵抗95D细胞中HIF?1α表达上调，侵袭转移相关蛋白VEGF和MMP?2表达下调。结论在人肺癌高转移细胞株95D失巢凋亡过程中，缺氧微环境能从一定程度上抑制失巢凋亡抵抗细胞的生长，缺氧微环境能降低失巢凋亡抵抗细胞的生存能力和转移能力，但这可能是缺氧后早期表现。
目的：研究缺氧微環境對人巨細胞肺癌高轉移細胞繫95D失巢凋亡的影響。方法對95D懸浮培養，建立人肺癌高轉移細胞株95D失巢凋亡牴抗細胞模型，實驗組進行缺氧培養，對照組正常有氧培養，MTT檢測缺氧微環境對于失巢凋亡牴抗95D細胞繫生長的影響，流式細胞儀檢測2組細胞的凋亡情況，Transwell實驗檢測2組細胞侵襲能力，Western blot檢測缺氧對于失巢凋亡牴抗95D細胞中HIF?1α、VEGF和MMP?2蛋白錶達的影響。結果缺氧微環境下失巢凋亡牴抗細胞組生長率為52.9%；凋亡率（40.4%）明顯高于非缺氧培養組（21.7%），差異有統計學意義（P<0.05）；侵襲能力降低，穿過膜細胞數（40.1±6.7）明顯低于對照組（12.5±7.9），差異有統計學意義（P<0.05）；缺氧微環境下失巢凋亡牴抗95D細胞中HIF?1α錶達上調，侵襲轉移相關蛋白VEGF和MMP?2錶達下調。結論在人肺癌高轉移細胞株95D失巢凋亡過程中，缺氧微環境能從一定程度上抑製失巢凋亡牴抗細胞的生長，缺氧微環境能降低失巢凋亡牴抗細胞的生存能力和轉移能力，但這可能是缺氧後早期錶現。
목적：연구결양미배경대인거세포폐암고전이세포계95D실소조망적영향。방법대95D현부배양，건립인폐암고전이세포주95D실소조망저항세포모형，실험조진행결양배양，대조조정상유양배양，MTT검측결양미배경대우실소조망저항95D세포계생장적영향，류식세포의검측2조세포적조망정황，Transwell실험검측2조세포침습능력，Western blot검측결양대우실소조망저항95D세포중HIF?1α、VEGF화MMP?2단백표체적영향。결과결양미배경하실소조망저항세포조생장솔위52.9%；조망솔（40.4%）명현고우비결양배양조（21.7%），차이유통계학의의（P<0.05）；침습능력강저，천과막세포수（40.1±6.7）명현저우대조조（12.5±7.9），차이유통계학의의（P<0.05）；결양미배경하실소조망저항95D세포중HIF?1α표체상조，침습전이상관단백VEGF화MMP?2표체하조。결론재인폐암고전이세포주95D실소조망과정중，결양미배경능종일정정도상억제실소조망저항세포적생장，결양미배경능강저실소조망저항세포적생존능력화전이능력，단저가능시결양후조기표현。
Objective To investigate the effect of hypoxia micro?environment on anoikis in human high?metastatic cell line 95D of giant cell carci?noma of lung. Methods Suspension technology was used to culture 95D to establish the model of anoikis?resistant 95D cells. Hypoxic culture was conducted in the experimental group,and regular culture was conducted in the control group. The effect of hypoxia on proliferation of anoikis?resis?tant 95D was investigated by MTT and the apoptosis in the two groups were detected by flow cytometer. The invasive ability of the cells was assessed by Transwell test. The effect of hypoxia on the expression of HIF?1α,VEGF and MMP?2 in anoikis?resistant 95D was detected by Western blot. Re?sults The growth rate of the anoikis?resistant 95D cells treated with hypoxia was 52.9%,and the apoptosis rate of these cells was higher than that in the non?hypoxic group(40.4%vs 21.7%,P<0.05). The treatment of hypoxia down?regulated the invasive ability,the number of migration cells un?der hypoxia was higher than that in the control group,with statistical significance(40.1±6.7 vs 12.5±7.9,P<0.05). The up?regulation of HIF?1α, the down?regulation of VEGF and MMP?2 were observed in the group of hypoxia. Conclusion During anoikis of human high?metastatic lung can?cer cell line 95D,hypoxia inhibited the survival ability and the metastasis ability of anoikis?resistant cells,which,however,might be the early mani?festation of hypoxia.
