中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2015年
5期
438-442
,共5页
崔苗苗%宋月佳%顾云鹤%戚基萍
崔苗苗%宋月佳%顧雲鶴%慼基萍
최묘묘%송월가%고운학%척기평
上皮间质转化标记蛋白%间质上皮转化%胃癌%淋巴结转移
上皮間質轉化標記蛋白%間質上皮轉化%胃癌%淋巴結轉移
상피간질전화표기단백%간질상피전화%위암%림파결전이
epithelial-to-mesenchymal transition markers%mesenchymal-to-epithelial transition%gastric cancer%lymph node metastasis
目的：探讨上皮间质转化（EMT）标志物在胃癌组织和转移淋巴结中的表达情况及其与恶性生物学行为的相关性。方法采用免疫组化法检测145例胃癌、25例异常增生、13例肠化、42例转移淋巴结及40例正常胃黏膜组织中EMT标志物E?cadherin、β?catenin、N?cadherin、转化生长因子β1（TGF?β1）及Snail的表达情况。结果胃癌组织中E?cadherin、β?catenin、N?cadherin、TGF?β1及Snail的阳性表达率分别为73.5%、65.5%、14.5%、53.1%、35.9%，正常胃黏膜组织中分别为100%、100%、0%、27.5%、2.5%，差异有统计学意义（P<0.05）。E?cadherin、β?catenin表达水平的降低及TGF?β1表达水平的升高与胃癌浸润程度有关（P<0.05）。E?cadherin与β?catenin的表达呈正相关，而与TGF?β1呈负相关（P<0.05）。N?cadherin与TGF?β1的表达呈正相关（P<0.05）。转移淋巴结中，E?cadherin和β?catenin的表达水平高于胃癌组织，而TGF?β1的表达低于胃癌组织（P<0.05）。结论 TGF?β1和Snail的高表达以及E?cadherin、β?catenin、N?cadherin的低表达与胃癌的浸润和转移有密切关系；在胃癌的发展过程中，E?cadherin向N?cadherin转化及TGF?β1表达可能发挥重要作用。淋巴结转移灶中出现了间质上皮转化（MET）。
目的：探討上皮間質轉化（EMT）標誌物在胃癌組織和轉移淋巴結中的錶達情況及其與噁性生物學行為的相關性。方法採用免疫組化法檢測145例胃癌、25例異常增生、13例腸化、42例轉移淋巴結及40例正常胃黏膜組織中EMT標誌物E?cadherin、β?catenin、N?cadherin、轉化生長因子β1（TGF?β1）及Snail的錶達情況。結果胃癌組織中E?cadherin、β?catenin、N?cadherin、TGF?β1及Snail的暘性錶達率分彆為73.5%、65.5%、14.5%、53.1%、35.9%，正常胃黏膜組織中分彆為100%、100%、0%、27.5%、2.5%，差異有統計學意義（P<0.05）。E?cadherin、β?catenin錶達水平的降低及TGF?β1錶達水平的升高與胃癌浸潤程度有關（P<0.05）。E?cadherin與β?catenin的錶達呈正相關，而與TGF?β1呈負相關（P<0.05）。N?cadherin與TGF?β1的錶達呈正相關（P<0.05）。轉移淋巴結中，E?cadherin和β?catenin的錶達水平高于胃癌組織，而TGF?β1的錶達低于胃癌組織（P<0.05）。結論 TGF?β1和Snail的高錶達以及E?cadherin、β?catenin、N?cadherin的低錶達與胃癌的浸潤和轉移有密切關繫；在胃癌的髮展過程中，E?cadherin嚮N?cadherin轉化及TGF?β1錶達可能髮揮重要作用。淋巴結轉移竈中齣現瞭間質上皮轉化（MET）。
목적：탐토상피간질전화（EMT）표지물재위암조직화전이림파결중적표체정황급기여악성생물학행위적상관성。방법채용면역조화법검측145례위암、25례이상증생、13례장화、42례전이림파결급40례정상위점막조직중EMT표지물E?cadherin、β?catenin、N?cadherin、전화생장인자β1（TGF?β1）급Snail적표체정황。결과위암조직중E?cadherin、β?catenin、N?cadherin、TGF?β1급Snail적양성표체솔분별위73.5%、65.5%、14.5%、53.1%、35.9%，정상위점막조직중분별위100%、100%、0%、27.5%、2.5%，차이유통계학의의（P<0.05）。E?cadherin、β?catenin표체수평적강저급TGF?β1표체수평적승고여위암침윤정도유관（P<0.05）。E?cadherin여β?catenin적표체정정상관，이여TGF?β1정부상관（P<0.05）。N?cadherin여TGF?β1적표체정정상관（P<0.05）。전이림파결중，E?cadherin화β?catenin적표체수평고우위암조직，이TGF?β1적표체저우위암조직（P<0.05）。결론 TGF?β1화Snail적고표체이급E?cadherin、β?catenin、N?cadherin적저표체여위암적침윤화전이유밀절관계；재위암적발전과정중，E?cadherin향N?cadherin전화급TGF?β1표체가능발휘중요작용。림파결전이조중출현료간질상피전화（MET）。
Objective To investigate the association between expression of the epithelial?to?mesenchymal transition(EMT)biomarkers and the malignant progression of gastric cancer in primary tumors and metastases and their possible correlation with progression of gastric cancer(GC). Methods The EMT biomarkers including E?cadherin,β?catenin,N?cadherin,Snail and TGF?β1 were detected by immunohistochemical method for 145 cases of gastric cancer(GC),25 cases of abnormal hyperplasia,13 cases of intestinal metaplasia,42 cases of lymph node metastasis and 40 cases of normal gastric mucosa tissues. Results Positive rates of TGF?β1,Snail,E?cadherin,β?catenin and N?cadherin were 73.5%,65.5%, 14.5%,53.1%and 35.9%,respectively,in gastric cancer tissues and 100%,100%,0%,27.5%and 2.5%,respectively,in normal gastric tissues, with a significant difference between the two groups(P<0.05). The decreased expression of E?cadherin andβ?catenin and the increased expression of TGF?β1 were related to the depth of invasion of gastric cancer(P<0.05). The expression of E?cadherin was correlated positively with the expres?sion ofβ?catenin,but negatively with the expression of TGF?β1. Whereas,the expression of N?cadherin was correlated positively with the expression of TGF?β1(P<0.05). The expression of E?cadherin andβ?catenin in lymph node metastasis was significantly higher than that in gastric cancer tis?sues,while the expression of TGF?β1 was lower than in gastric cancer tissues(P<0.05). Conclusion The increased expression of TGF?β1 and Snail and the decreased expression of E?cadherin,β?catenin,and N?cadherin are involved in the processes of invasion and metastasis of GC. The transformation of E?cadherin to N?cadherin and the expression of TGF?β1 may play an important role in the development of GC. In lymph node me?tastasis,the phenomenon of mesenchymal?to?epithelial transition(MET)occurs.
