CAJ | 학술논문

目的：研究将氧化型甘露聚糖修饰的肾透明细胞癌细胞786?0作为肿瘤细胞抗原致敏树突状细胞（DC），观察其诱导产生的CTL对肾透明细胞癌的杀伤作用。方法分离健康志愿者外周血单个核细胞，体外经GM?CSF和IL?4刺激DC成熟。以肾癌786?0整个细胞作为肿瘤抗原，并制备成氧化型甘露聚糖肿瘤细胞疫苗。实验分组：空白组（DC?PBS组），对照组（Control?DC?786?0融合瘤苗组）、实验组（DC?Ox?Mannose?786?0融合瘤苗组）。流式细胞术检测各组DC表型的变化情况，ELISA法检测各组DC上清IL?12的分泌水平，CCK?8法检测各组DC诱导的细胞毒性T淋巴细胞（CTLs）对肾癌细胞786?0的杀伤效果。结果经流式细胞仪分析检测：DC?Ox?M?786?0组DC表面成熟标志CD80、CD83、CD86、HLA?DR的表达水平明显高于Control?DC?786?0组及DC?PBS组，ELISA法测得实验组IL?12的分泌水平也明显高于Control?DC?786?0组和DC?PBS组，与此同时DC?Ox?M－786?0组诱导的各组细胞毒性T淋巴细胞（CTLs）杀瘤活性较Control?DC?786?0组、DC?PBS组均明显增强。结论氧化型甘露聚糖化抗原能更为有效的致敏DC，并刺激其成熟，增强其抗原递呈功能，与此同时对于肿瘤细胞的杀伤作用也有明显的提高。
목적：연구장양화형감로취당수식적신투명세포암세포786?0작위종류세포항원치민수돌상세포（DC），관찰기유도산생적CTL대신투명세포암적살상작용。방법분리건강지원자외주혈단개핵세포，체외경GM?CSF화IL?4자격DC성숙。이신암786?0정개세포작위종류항원，병제비성양화형감로취당종류세포역묘。실험분조：공백조（DC?PBS조），대조조（Control?DC?786?0융합류묘조）、실험조（DC?Ox?Mannose?786?0융합류묘조）。류식세포술검측각조DC표형적변화정황，ELISA법검측각조DC상청IL?12적분비수평，CCK?8법검측각조DC유도적세포독성T림파세포（CTLs）대신암세포786?0적살상효과。결과경류식세포의분석검측：DC?Ox?M?786?0조DC표면성숙표지CD80、CD83、CD86、HLA?DR적표체수평명현고우Control?DC?786?0조급DC?PBS조，ELISA법측득실험조IL?12적분비수평야명현고우Control?DC?786?0조화DC?PBS조，여차동시DC?Ox?M－786?0조유도적각조세포독성T림파세포（CTLs）살류활성교Control?DC?786?0조、DC?PBS조균명현증강。결론양화형감로취당화항원능경위유효적치민DC，병자격기성숙，증강기항원체정공능，여차동시대우종류세포적살상작용야유명현적제고。
Objective To study to take the oxidized Mannan?modified 786?0 in renal clear cell carcinom as tumor cells antigen to sensitize Dendrit?ic cells(DC)and to observe the its killing effect on renal clear cell carcinoma of CTLs induced. Methods Getting the peripheral blood mononucle?ar cells from the volunteers,and then to be stimulated to turn to be maturation by GM?CSF and IL?4 in vitro. Taking the clear renal carcinoma cell as the tumor antigen,and then making it to be modified by oxidized Mannan to acquire the tumor cell vaccine.Experimental groups include:blank group:DC?PBS group,control group:control?DC?786?0,experimental group:DC?Ox?Mannose?786?0 group. Taking the flow cytometry to detect the changes of DC phenotype,then taking the ELISA to detect the sencretion levels of supernatant of IL?12 of DC,then taking the CCK to detecte the cytotoxicity of lymphocytes(CTL)induced by DC of these experiment groups. Results Results by flow cytometry:the mature phenotype of DCs sensitized by Ox?Mannose?786?0 group included CD80,CD83,CD86 and HLA?DR expressed significantly higher than the other groups. As well as the secretion levels of IL?12. Meanwhile the cytotoxicity activity of lymphocytes(CTLs)induced by DCs which are sensitized by Ox?Mannose?786?0 increased more significantly than the other groups. Conclusion Glycosylated Antigens can be more effective in sensitizing antigen?presenting cells DC,and stimulating them to be maturation,while the killing effect to tumor cells also have noticeably improved.