青海大学学报（自然科学版）
青海大學學報（自然科學版）
청해대학학보（자연과학판）
JOURNAL OF QINGHAI UNIVERSITY (NATURAL SCIENCE EDITION)
2015年
2期
6-11
,共6页
PCR-DGGE%户用沼气池%微生物群落%优化%青海
PCR-DGGE%戶用沼氣池%微生物群落%優化%青海
PCR-DGGE%호용소기지%미생물군락%우화%청해
PCR-DGGE%household biogas digesters%microbial community%optimization%Qinghai
采用变性梯度凝胶电泳技术研究青海农村户用沼气池微生物群落，对其PCR－DGGE的电泳时间及上样量进行优化。结果表明：最佳电泳时间为16 h，最适上样量为4μg，优化后的PCR－DGGE确保了实验的准确性和可重复性。运用此优化后的PCR－DGGE技术对2个户用沼气池内细菌和古菌群落进行了研究，获得了较丰富的多样性。
採用變性梯度凝膠電泳技術研究青海農村戶用沼氣池微生物群落，對其PCR－DGGE的電泳時間及上樣量進行優化。結果錶明：最佳電泳時間為16 h，最適上樣量為4μg，優化後的PCR－DGGE確保瞭實驗的準確性和可重複性。運用此優化後的PCR－DGGE技術對2箇戶用沼氣池內細菌和古菌群落進行瞭研究，穫得瞭較豐富的多樣性。
채용변성제도응효전영기술연구청해농촌호용소기지미생물군락，대기PCR－DGGE적전영시간급상양량진행우화。결과표명：최가전영시간위16 h，최괄상양량위4μg，우화후적PCR－DGGE학보료실험적준학성화가중복성。운용차우화후적PCR－DGGE기술대2개호용소기지내세균화고균군락진행료연구，획득료교봉부적다양성。
The microbial community in rural household biogas digesters of Qinghai was investigated in this study with PCR -DGGE technology .In order to improve the accuracy and repeatability of DGGE, the running parameters of DGGE were optimized by electrophoresis time and sampling a-mount, respectively.The results showed that the optimal running time was 16 hours, and the sam-pling amount was 4μg.Furthermore, bacterial community and archaeal community from two rural household biogas digesters were also performed by using these optimized reaction conditions .It was found that the diversity of bacteria and archaea were rich .
