CAJ | 학술논문

目的：探讨shRNA介导的XRCC2基因沉默联合辐射对结肠癌细胞移植裸鼠肿瘤的疗效。方法将结肠癌T84细胞接种于BALB/c裸鼠建立肿瘤移植模型，分为control组、shRNA?SC组和shRNA?XRCC2组。裸鼠经X射线局部照射，检测放射治疗后肿瘤的体积和质量变化，并对肿瘤组织进行病理分析。结果 shRNA?XRCC2组肿瘤生长速度较缓慢，从放射治疗的第12天开始至放射治疗继续到第28天，shRNA?XRCC2组瘤体明显较control组减小，差异有统计学意义（P<0.01）。shRNA?XRCC2组肿瘤质量明显轻于control组，二者比较差异有统计学意义（t=18.843，P<0.01）。shRNA?XRCC2组裸鼠移植瘤的抑瘤率为56.25%，远远高于shRNA?SC组（4.69%）。移植瘤组织的病理检查结果表明，shRNA?XRCC2组肿瘤组织核异型不明显，少见核分裂相，多见坏死区。结论 XRCC2基因沉默联合辐射对裸鼠结肠癌移植瘤具有显著的抑瘤效果。
목적：탐토shRNA개도적XRCC2기인침묵연합복사대결장암세포이식라서종류적료효。방법장결장암T84세포접충우BALB/c라서건립종류이식모형，분위control조、shRNA?SC조화shRNA?XRCC2조。라서경X사선국부조사，검측방사치료후종류적체적화질량변화，병대종류조직진행병리분석。결과 shRNA?XRCC2조종류생장속도교완만，종방사치료적제12천개시지방사치료계속도제28천，shRNA?XRCC2조류체명현교control조감소，차이유통계학의의（P<0.01）。shRNA?XRCC2조종류질량명현경우control조，이자비교차이유통계학의의（t=18.843，P<0.01）。shRNA?XRCC2조라서이식류적억류솔위56.25%，원원고우shRNA?SC조（4.69%）。이식류조직적병리검사결과표명，shRNA?XRCC2조종류조직핵이형불명현，소견핵분렬상，다견배사구。결론 XRCC2기인침묵연합복사대라서결장암이식류구유현저적억류효과。
Objective To study the curative effect of XRCC2 gene silencing mediated by shRNA combined with radiation on human colonic trans?planted carcinoma in nude mice. Methods Colonic carcinoma T84 cells were transfered into BALB/c nude mice to establish a tumor xenograft mod?el in vivo. Mice were divided into three groups:control,shRNA?SC and shRNA?XRCC2 and exposed to X?ray radiation. The change of volume and weight of the xenografts were examined after receiving radiotherapy and the pathological analysis of tumor tissues were conducted. Results Tumor xenografts transfected with shRNA?XRCC2 in nude mice grew slowly. The xenograft volume in the shRNA?XRCC2 group was decreased significant?ly from day 12 to day 28 after radiotherapy compared with the control group(P<0.01). The xenograft weight in the shRNA?XRCC2 group was small?er than in the control group,with statistically significant difference(t=18.843,P<0.01). The inhibited rate of xenografts in the shRNA?XRCC2 group(56.25%),was markedly higher than that in the shRNA?SC group(4.69%). Pathological analysis of colonic transplanted carcinoma showed that nuclear atypia was not obvious,karyokinesis was decreased and small areas of necrosis were present in tumor xenografts treated with shRNA?XRCC2 transfection. Conclusion XRCC2 gene silencing combined with radiation has significant inhibition effect on colonic transplanted carcino?ma in nude mice.