CAJ | 학술논문

针对焦化废水生物难降解有机物含量高、实际生产过程中的水质和水量变化大的特点，开展了高效菌株处理含酚废水的试验研究。从某焦化厂水处理车间生物处理装置曝气池活性污泥中驯化、分离和筛选得到4株以苯酚为唯一碳源的高效降解菌，并对其进行鉴定及降解特性研究。结果表明：初步鉴定h32a2、b31B、h31A和b41a为假单胞菌属（ Pseudomonas sp．）；通过溴化容量法测定苯酚含量，确定了菌株的最佳降解条件为：温度32℃，pH值7.5，培养时间16 h，接种密度1％，且单一菌株h32a2的苯酚最大降解率可达90.55％。通过单一和组合高效菌株对苯酚的降解特性可知，4株高效菌混合的降解效果最佳。因此，混合高效苯酚降解菌处理含酚废水具有一定的可行性。
침대초화폐수생물난강해유궤물함량고、실제생산과정중적수질화수량변화대적특점，개전료고효균주처리함분폐수적시험연구。종모초화엄수처리차간생물처리장치폭기지활성오니중순화、분리화사선득도4주이분분위유일탄원적고효강해균，병대기진행감정급강해특성연구。결과표명：초보감정h32a2、b31B、h31A화b41a위가단포균속（ Pseudomonas sp．）；통과추화용량법측정분분함량，학정료균주적최가강해조건위：온도32℃，pH치7.5，배양시간16 h，접충밀도1％，차단일균주h32a2적분분최대강해솔가체90.55％。통과단일화조합고효균주대분분적강해특성가지，4주고효균혼합적강해효과최가。인차，혼합고효분분강해균처리함분폐수구유일정적가행성。
According to the characteristics that there are high content of bio-refractory organic matters in the coking wastewater and the changes of water quality and water quantity during actual production are great, the authors of this paper mainly studied the treatment of wastewater containing phenol by high efficient strains.four highly efficient degradation bacteria strains which take phenol as the sole carbon source were obtained by means of domestication, isolation and screening to the activated sludge in the aeration tank, a biological treatment device, of the water treatment plant in a coking factory.Those four highly efficient degradation bacteria strains were identified and its degradation characteristics were studied.The results show that the strain h32a2, b31B, h31A and b41a are identified as Pseudomonas sp;Phenol content was measured by brominating volumetric method.The best condition of strain degradation was determined as follows: the degradation temperature was 32℃, the pH value is 7.5 , the strain incubation time was 16h, the inoculation density was 1%, and the maximum phenol degradation rate of the strain h32 a2 reached 90.55%. We can know that the degradation effect of combined four highly efficient degradation bacteria strains is the best from the degradation characteristics of single and combined strain.The treatment of wastewater containing phenol by mixed high efficient phenol-degradation bacteria was feasible.